Abstract
Female rats were given a low-calcium diet (0.05%) during the last three weeks of the lactating period, followed by a normal diet (1.03% calcium) during the first three weeks after lactation. The resulting bone loss and its recovery were studied by means of microradiography, tetracycline-uptake, quantitative estimation of the cortical area of cross-sections from the femoral midshaft, and estimation of total body calcium. The cortical area in the femoral midshaft fell to 46% of its original value during depletion, and then rose to 78% during the first three weeks after weaning. Total body calcium fell from 1.12% to 0.60% of body weight and then increased to 0.89%. Removal of bone occurred mainly in the spongiosa and on the endosteal side of the cortex. Subsequently, new bone was laid down on the endosteal side, but also to some extent on the periosteal side of the cortex. The mineral density of this new bone was low. During the recovery phase resorption cavities within the cortex were filled in a concentric manner as in Haversian remodeling. Neither this feature nor the low mineral density of bone are normally present in the rat.
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Ellinger, G. M., Duckworth, J., Dalgarno, A. C., Quenouille, M. H.: Skeletal changes during pregnancy and lactation in the rat: Effect of different levels of dietary calcium. Brit. J. Nutr.6, 235–253 (1952)
Fournier, P., Surbielle, H.: Les échanges de calcium chez le rat au cours de la gestation, de la lactation et du sévrage. I: Influence d’une régime riche en calcium. J. Physiol. (Paris)44, 123–134 (1952)
Fournier, P., Surbielle, H.: Les échanges de calcium chez le rat au cours de la gestation, de la lactation et du sévrage. II: Influence d’une régime pauvre en calcium. J. Physiol. (Paris)44, 575–590 (1952)
Garn, S. M. The earlier gain and the later loss of cortical bone, p. 19. Springfield: C.C. Thomas 1970.
Romeis, B.: Mikroskopische Technik, 16th ed., p. 80. München-Wien: Oldenbourg Verlag 1968
Ruth, E. B.: Bone studies. II. An experimental study of the Haversian-type vascular channels. Amer. J. Anat.93, 429–455 (1953).
Sevastikoglou, J. A., Larsson, S. E.: Osteoporosis and the parathyroid glands. I: The effect of prolonged calcium deficiency on the parathyroids of the adult rat. Clin. Orthop. Rel. Res.85, 163–170 (1972)
Stauffer, M., Baylink, D., Wergedal, J., Rich, C.: Bone repletion in calcium deficient rats fed a high calcium diet. Calcif. Tiss. Res.9, 163–172 (1972)
Taussky, L. A., Shorr, E. A.: A microcolorimetric method for the determination of inorganic phosphorous. J. biol. Chem.202, 675–685 (1953)
Widdowson, E. M., McCance, R. A.: The metabolism of calcium, phosphorus, magnesium and strontium. Ped. Clin. N. Amer.12, 595–614 (1965).
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de Winter, F.R., Steendijk, R. The effect of a low-calcium diet in lactating rats; Observations on the rapid development and repair of osteoporosis. Calc. Tis Res. 17, 303–316 (1975). https://doi.org/10.1007/BF02546602
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DOI: https://doi.org/10.1007/BF02546602