Abstract
Oxalate is metabolized by the glycerate pathway involving glyoxylate carboligase inAlcaligenes LOx andPseudomonas KOx, and by the serine pathway involving hydroxypyruvate reductase inPs.MOx andPs.AM1 (var. 470).
AlthoughA.LOx does not grown on formate, stimulation of growth was observed in the presence of amino acids and a few Kreb's cycle intermediates.A.LOx possesses two different mechanisms for the oxidation of formate: (1) the constitutive formate oxidase which is present in the particulate fraction of oxalate-grown and succinate-plus-formate-grown cells; (2) the inducible NAD-linked formate dehydrogenase present in the 100000×g supernatant fraction of the cell-free extracts of oxalate-grown cells alone. The two systems occur simultaneously in oxalate-grown cells. The effect of inhibitors on formate oxidase activity and the other enzyme activities of the particulate formate-oxidizing fraction indicate that the oxidation of formate is linked to the respiratory chain.
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References
Anthony, C. andZatman, L. J. 1964. The microbial oxidation of methanol. 1. Isolation and properties ofPseudomonas sp. M.27.—Biochem. J.92: 609–614.
Blackmore, M. A. andQuayle, J. R. 1968. Choice between autotrophy and heterotrophy inPseudomonas oxalaticus. Growth in mixed substrates.—Biochem. J.107: 705–713.
Blackmore, M. A. andQuayle, J. R. 1970. Microbial growth on oxalate by a route not involving glyoxylate carboligase.—Biochem. J.118: 53–59.
Chandra, T. S. 1974. Oxalate and formate metabolism in anAlcaligenes species. Abstract MB-14;—15th Annual Conference of the Association of Microbiologists of India.
Chandra, T. S. andShethna, Y. I. 1975. Isolation and characterization of some new oxalate-decomposing bacteria.—Antonie van Leeuwenhoek41: 101–111.
Gale, E. F. 1939. Formic dehydrogenase ofBacterium coli: Its inactivation by oxygen and its protection in the bacterial cell.—Biochem. J.,33: 1012–1027.
Heinen, W. 1971. Inhibitors of electron transport and oxidative phosphorylation, p. 383–393.In J. R. Norris and D. W. Ribbons, (eds.), Methods in Microbiology, Vol. 6A.—Academic Press, New York.
Iida, K. andTaniguchi, S. 1959. Studies on nitrate reductase system ofEscherichia coli. I. Particulate electron transport system to nitrate and its solubilization.—J. Biochem.46: 1041–1055.
Johnson, P. A., Jones-Mortimer, M. C. andQuayle, J. R. 1964. Use of a purified bacterial formate dehydrogenase for the micro-estimation of formate.—Biochim. Biophys. Acta89: 351–353.
Kaltwasser, H. 1968. Harnsäureabbau und Biosynthese der Enzyme Uricase, Glyoxylatcarboligase und Urease beiHydrogenomonas H16. 1. Bildung von Glyoxylatcarboligase undd-Glycerat-3-Dehydrogenase.—Arch. Mikrobiol.64: 71–84.
Kornberg, H. L. 1966. Anaplerotic sequences and their role in metabolism, p. 1–31.In P. N. Campbell and G. D. Greville, (eds.), Essays in Biochemistry, Vol. 2.—Academic Press, London and New York.
Kornberg, H. L. andElsden, S. R. 1961. The metabolism of 2-carbon compounds by microorganisms. —Advan. Enzymol.23: 401–470.
Kung, H.-F. andWagner, C. 1970. Oxidation of C1 compounds byPseudomonas sp. MS.— Biochem. J.116: 357–365.
Large, P. J. andQuayle, J. R. 1963. Microbial growth on C1 compounds. 5. Enzyme activities in extracts ofPseudomonas AM1.—Biochem. J.87: 386–396.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent.—J. Biol. Chem.193: 265–275.
Malavolta, E., Delwiche, C. C. andBurge, W. D. 1962. Formate oxidation by cell-free preparations fromNitrobacter agilis.—Biochim. Biophys. Acta57: 347–351.
O'Kelley, J. C. andNason, A. 1970. Particulate formate oxidase fromNitrobacter agilis.— Biochim. Biophys. Acta205: 426–436.
Quayle, J. R. 1963a. Carbon assimilation byPseudomonas oxalaticus (OX1). 6. Reactions of oxalyl-coenzyme A.—Biochem. J.87: 368–373.
Quayle, J. R. 1963b. Carbon assimilation byPseudomonas oxalaticus (OX1). 7. Decarboxylation of oxalyl-coenzyme A to formyl-coenzyme A.—Biochem. J.,89: 492–503.
Quayle, J. R. 1972. The metabolism of one-carbon compounds by micro-organisms.— Advan. Microbial Physiol.7: 119–203.
Quayle, J. R., Keech, D. B. andTaylor, G. A. 1961. Carbon assimilation byPseudomonas oxalaticus (OX1). 4. Metabolism of oxalate in cell-free extracts of the organism grown on oxalate.—Biochem. J.78: 225–236.
Quayle, J. R. andTaylor, G. A. 1961. Carbon assimilation byPseudomonas oxalaticus (OX1). 5. Purification and properties of glyoxylic dehydrogenase.—Biochem. J.78: 611–615.
Radcliffe, B. C. andNicholas, D. J. D. 1970. Some properties of a nitrate reductase fromPseudomonas denitrificans.—Biochim. Biophys. Acta205: 273–287.
Sanadi, D. R. andJacobs, E. E. 1967. Assay of oxidative phosphorylation at the cytochrome oxidase region (site III) p. 38–41.—In S.P. Colowick and N. O. Kaplan, (eds.), Methods in Enzymology, Vol. X.—Academic Press, Inc., New York.
Smith, L. 1961. Cytochrome systems in aerobic electron transport, p. 365–396. (p. 375).In I. C. Gunsalus and R. Y. Stanier, (eds.), The Bacteria. Vol. 2.—Academic Press, New York.
Taniguchi, S. andItagaki, E. 1960. Nitrate reductase of nitrate respiration type fromE. coli. 1. Solubilization and purification from the particulate system with molecular characterization as a metalloprotein.—Biochim. Biophys. Acta44: 263–279.
Umbreit, W. W., Burris, R. H. andStauffer, J. F. 1964. Manometric techniques, 4th ed. p. 28.—Burgess Publishing Company, Minnesota.
Van Gool, A. andLaudelout, H. 1966. Formate utilization byNitrobacter winogradskyi.— Biochim. Biophys. Acta127: 295–301.
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Chandra, T.S., Shethna, Y.I. Oxalate and formate metabolism inAlcaligenes andPseudomonas species. Antonie van Leeuwenhoek 41, 465–477 (1975). https://doi.org/10.1007/BF02565090
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DOI: https://doi.org/10.1007/BF02565090