Summary
Antisera to six mammalian pituitary hormones and a synthetic corticotrophin (Synacthen) were prepared and tested for specificity. The gamma-globulin fractions of the antisera were conjugated with fluorescein isothiocyanate and the labelled antibodies were isolated by column chromatography.
Fresh-frozen sections of pituitary glands of adult migratory sockeye salmon (Oncorhynchus nerka) were incubated with the antibody solutions and examined with a fluorescence microscope. Anti-ACTH and anti-Synacthen appeared to be bound by theepsilon cells, whereas anti-prolactin reacted with theeta cells. Anti-GH was bound to the acidophil cells of the proximal pars distalis. Anti-LH reacted with some of the basophil cells of the proximal pars distalis. Antibodies to FSH or TSH failed to react.
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We are most grateful to Dr. E. W. Emmart, National Institutes of Health, Bethesda, Maryland, for her stimulating help. We would also like to express our gratitude to Messrs. G. Alexander and R. Calland for technical assistance. We acknowledge the assistance of Mr. J. Weir, Mr. J. Roos, Mr. B. Davis, and in particular Mr. S. Killick of the International Pacific Salmon Fisheries Commission, as well as Mr. J. Harvey of the Federal Department of Fisheries for the collection of salmon. We would like to extend our appreciation to Dr. C. W. Murphy of Ciba for his kind gift of Synacthen. Thanks are also due to the Endocrinology Study Section, National Institutes of Health, Bethesda, Maryland, for their kind gift of purified ovine pituitary hormones.
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McKeown, B.A., van Overbeeke, A.P. Immunohistochemical identification of pituitary hormone producing cells in the sockeye salmon (Oncorhynchus nerka, Walbaum). Z.Zellforsch 112, 350–362 (1970). https://doi.org/10.1007/BF02584048
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DOI: https://doi.org/10.1007/BF02584048