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In vitro conversion of proinsulin to insulin by cathepsin B and role of C-peptide

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Summary

Cathepsin B, purified from isolated islets of Langerhans, when incubated with proinsulin underin vitro conditions could convert proinsulin to insulin and C-peptide, releasing free arginine and lysine. When C-peptide, prepared from rat pancreas, was added to the incubation system consisting of proinsulin and cathepsin B, it completely inhibited the conversion of proinsulin to insulin.

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Authors and Affiliations

  1. Division of Biochemistry, Central Drug Research Institute, 226001, Lucknow, India

    Renu Bala Puri, Kawluru Anjaneyulu, Jalil R. Kidwai & Varanasi K. Mohan Rao

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  1. Renu Bala Puri
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  2. Kawluru Anjaneyulu
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  3. Jalil R. Kidwai
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  4. Varanasi K. Mohan Rao
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Communication no. 2295 from the Central Drug Research Institute, Lucknow, India.

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Puri, R.B., Anjaneyulu, K., Kidwai, J.R. et al. In vitro conversion of proinsulin to insulin by cathepsin B and role of C-peptide. Acta diabet. lat 15, 243–250 (1978). https://doi.org/10.1007/BF02590747

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  • DOI: https://doi.org/10.1007/BF02590747

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