Skip to main content
SpringerLink
Log in

Characterization of Spiroplasmas by one- and two-dimensional protein analysis on polyacrylamide slab gels

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Differences betweenSpiroplasma citri isolates were detected by one-dimensional electrophoresis of proteins on gradient polyacrylamide slab gels. Two-dimensional protein maps (electrofocusing followed by electrophoresis) showed a highly characteristic pattern for allS. citri isolates examined. Coanalysis of mixed protein samples from pairs ofS. citri strains revealed more than 150 comigrating proteins common to allS. citri isolates, but also a number of noncomigrating proteins. Some noncomigrating proteins were present in one isolate but not in another, while other proteins whose migrational properties were only slightly different from one isolate to the other (homologous proteins), were present in more than one isolate.S. citri isolates had many common and only a few homologous proteins. Comparisons ofS. citri with the corn stunt spiroplasma revealed few common proteins and a large number of homologous proteins. When comparingS. citri and the suckling mouse cataract spiroplasma, few common and homologous proteins were apparent. However, several of these common proteins were also shared by the corn stunt spiroplasma, suggesting that they may well represent genus-specific proteins. The data also offer additional evidence that the suckling mouse cataract spiroplasma differs significantly fromS. citri and corn stunt spiroplasmas and probably deserves a separate species designation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Bonner, W. M., Laskey, R. A. 1974. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry46:83–88.

    Article  PubMed  CAS  Google Scholar 

  2. Bové, J. M., Vignault, J. C., Garnier, M., Saillard, C., Garcia-Jurado, O., Bové, C., Nhami, A. 1978. Mise en évidence deSpiroplasma citri, l'agent causal de la maladie du “stubborn” des agrumes, dans des pervenches (Vinca rosea) ornementales de la ville de Rabat, Maroc. Comptes Rendus de l'Académie des Sciences. D286:57–59.

    Google Scholar 

  3. Chen, T. A., Liao, C. H. 1975. Corn stunt spiroplasma: Isolation, cultivation and proof of pathogenicity. Science188:1015–1017.

    Article  Google Scholar 

  4. Daniels, M. J., Meddins, B. M. 1973. Polyacrylamide gel electrophoresis of mycoplasma proteins in sodium-dodecyl-sulfate. Journal of General Microbiology76:239–242.

    PubMed  CAS  Google Scholar 

  5. Giannotti, M. J. 1974. Cultures de mollicutes à partir de plantes atteintes de jaunisse et d'insectes vecteurs. Les mycoplasmes. Colloques de l'Institut National de la Santé et de la Recherche Médicale, Bordeaux33:99–106.

    Google Scholar 

  6. Laemli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685.

    Article  Google Scholar 

  7. Mouches, C., Duthil, P., Vignault, J. C., Saillard, C., Bové, J. M. 1978. Mapping of spiroplasma proteins by high resolution one and two dimensional polyacrylamide gel analysis. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abt. I Orig., Reihe A241:221.

    Google Scholar 

  8. O'Farrell, P. 1975. High resolution two dimensional electrophoresis of proteins. Journal of Biological Chemistry250:4007–4021.

    PubMed  Google Scholar 

  9. Razin, S. 1968. Mycoplasma taxonomy studied by electrophoresis of cell proteins. Journal of Bacteriology96:687–694.

    PubMed  CAS  Google Scholar 

  10. Razin, S., Rottem, S. 1967. Identification ofMycoplasma and other micro-organisms by polyacrylamide gel electrophoresis of cell proteins. Journal of Bacteriology94:1807–1810.

    PubMed  CAS  Google Scholar 

  11. Rodwell, A. W., Archer, D. B., Peterson, J. E., Rodwell, E. S. 1978. The protein of mycoplasmas studied by two-dimensional gel electrophoresis. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abt. 1 Orig., Reihe A241:178–179.

    Google Scholar 

  12. Tully, J. G., Whitcomb, R. F., Clark, H. F., Williamson, D. L. 1977. Pathogenic mycoplasmas: Cultivation and vertebrate pathogenicity of a new spiroplasma. Science195:892–894.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire et Moleculaire, INRA, Domaine de la Grande Ferrade, F-33140, Pont de la Maye, France

    C. Mouches, J. C. Vignault & J. M. Bové

  2. University of Bordeaux II, Domaine de la Grande Ferrade, F-33140, Pont de la Maye, France

    C. Mouches, J. C. Vignault & J. M. Bové

  3. Laboratory of Infectious Disease, National Institute of Allergy and Infectious Diseases, 20014, Bethesda, Maryland, USA

    J. G. Tully

  4. Plant Protection Institute, Federal Research, Science and Education Administration, U.S. Department of Agriculture, 20014, Beltsville, Maryland, USA

    R. F. Whitcomb

Authors
  1. C. Mouches
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. C. Vignault
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. G. Tully
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. F. Whitcomb
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. M. Bové
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mouches, C., Vignault, J.C., Tully, J.G. et al. Characterization of Spiroplasmas by one- and two-dimensional protein analysis on polyacrylamide slab gels. Current Microbiology 2, 69–74 (1979). https://doi.org/10.1007/BF02603058

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02603058

Keywords

Search

Navigation