Summary
An agar medium has been developed which permits formation of colonies from cells of the insect cell lineTrichoplusia ni, with a plating efficiency of 1%. This technique has been successfully applied in the cloning of theT. ni line and is applicable to other insect lines displaying this property. Morphology, growth curves, and population doubling times of several clones are presented.
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This research was supported in part by National Science Foundation Grant GB29277X.
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McIntosh, A.H., Rechtoris, C. Insect cells: Colony formation and cloning in agar medium. In Vitro 10, 1–5 (1974). https://doi.org/10.1007/BF02615331
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DOI: https://doi.org/10.1007/BF02615331