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Pyruvate promotion of DNA synthesis in serum-free primary cultures of adult rat hepatocytes

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Summary

Pyruvate (2 to 60 mM), acting alone and in conjunction with insulin and epidermal growth factor (EGF), enhances DNA synthesis in primary monolayer cultures of adult rat liver parenchymal cells. Lactate can replace pyruvate in stimulating DNA synthesis. Several other intermediary metabolites (oxaloacetate, α-ketoglutarate, α-ketobutyrate, succinate, fumarate, and malate), though less potent than pyruvate and lactate, also elevate DNA synthesis, whereas alanine at similar concentrations is inhibitory.

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Authors and Affiliations

  1. Cell Biology Unit, Shriners Burns Institute, Surgical Services, Massachusetts General Hospital, 51 Blossom Street, 02114, Boston, Massachusetts

    Joan A. McGowan & Nancy L. R. Bucher

  2. Department of Surgery, Harvard Medical School, 51 Blossom Street, 02114, Boston, Massachusetts

    Joan A. McGowan & Nancy L. R. Bucher

Authors
  1. Joan A. McGowan
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  2. Nancy L. R. Bucher
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Additional information

This work is supported by U.S. Public Health Service Grant AM-19435 and Grant 15851 from the Shriners Hospital for crippled Children. Animals used in this study were maintained in accordance with the guidelines of the Committee on Animals of the Harvard Medical School and those prepared by the Committee on, Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources, National Research Council (DHEW publication (NIH) 78-23, revised 1978).

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McGowan, J.A., Bucher, N.L.R. Pyruvate promotion of DNA synthesis in serum-free primary cultures of adult rat hepatocytes. In Vitro 19, 159–166 (1983). https://doi.org/10.1007/BF02618054

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  • DOI: https://doi.org/10.1007/BF02618054

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