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Acid phosphatase: Endpoint for in vitro toxicity tests

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References

  1. Maehara, Y.; Anai, H.; Tamada, R., et al. The ATP assay is more sensitive than the succinnate dehydrogenase inhibition test for predicting cell viability. Eur. J. Cancer Clin. Oncol. 23:173–176; 1987.

    Google Scholar 

  2. Matthews, N.; Neale, M. L. Cytotoxicity assays for tumour necrosis factor and lymphotoxin. In: Clemins, M. J.; Morris, A. G.; Gearing, A. J. H., eds. Lymphokines and interferons: a practical approach. Oxford, Washington D.C.; IRL Press: 1987:222–225.

    Google Scholar 

  3. Twentyman, P. R.; Luscome, M. A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity. Br. J. Cancer 56:279–285; 1987.

    PubMed  CAS  Google Scholar 

  4. Scudiero, D. A.; Shoemaker, R. H.; Paull, D. K., et al. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res. 48:4827–4833; 1988.

    PubMed  CAS  Google Scholar 

  5. Borenfreund, E.; Puerner, J. A. A simple quantitative procedure using monolayer cultures for cytotoxicity assays. J Tissue Culture Methods 9:7–9; 1983.

    Article  Google Scholar 

  6. Huschtscha, L. I.; Lucibello, F. C.; Bodmer, W. F. A rapid method for counting cells ‘in situ’ using a fluorogenic alkaline phosphatase enzyme assay. In Vitro Cell. Devel. Biol. 25:105–108; 1989.

    Article  CAS  Google Scholar 

  7. Connolly, D. T.; Knight, M. B.; Harakas, N. K., et al. Determination of the number of endothelial cells in culture using an acid phosphatase assay. Anal. Biochem. 152:136–140; 1986.

    Article  PubMed  CAS  Google Scholar 

  8. Carmichael, J.; De Graff, W.; Gazdar, A. F., et al. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47:936–942; 1987.

    PubMed  CAS  Google Scholar 

  9. Hertz, F. Caution about assay of alkaline phosphatase to quantitate cell numbers. In Vitro Cell. Devel. Biol. 25:865; 1989.

    Article  Google Scholar 

  10. Jabbar, S. A. B.; Twentyman, P. R.; Watson, J. V. The MTT assay underestimates the growth inhibitory effects of interferons. Br. J. Cancer 60:523–528; 1989.

    PubMed  CAS  Google Scholar 

  11. Skehan, P.; Storeng, R.; Scuderio, D., et al. New colorimetric cytotoxicity assay for anticancer-drug screening. Journal of the National Cancer Institute 82:1107–1112; 1990.

    Article  PubMed  CAS  Google Scholar 

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Martin, A., Clynes, M. Acid phosphatase: Endpoint for in vitro toxicity tests. In Vitro Cell Dev Biol - Animal 27, 183–184 (1991). https://doi.org/10.1007/BF02630912

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