Skip to main content
SpringerLink
Log in

A modified CTAB DNA extraction procedure for plants belonging to the family proteaceae

  • Commentary
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

This paper describes rapid and efficient DNA extraction methods for mature leaves, resting buds and seedling leaves of genera in the family Proteaceae. The procedures combine and modify previously published techniques. The DNA can be digested by restriction endonucleases and is suitable for subsequent PCR amplification.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Couch, J.A. and P.J. Fritz. 1990. Isolation of DNA from plants high in polyphenolics. Plant Mol. Biol. Reptr. 8:8–12.

    Article  CAS  Google Scholar 

  • Do, N. and R.P. Adams. 1991. A simple technique for removing plant polysaccharide contaminants from DNA. Biotechniques 10:163–166.

    Google Scholar 

  • Doyle, J.J. and J.L. Doyle. 1988. Isolation of plant DNA from fresh tissue. Focus 12:13–15.

    Google Scholar 

  • Fang, G., S. Hammar and R. Grumet. 1992. A quick inexpensive method for removing polysaccharides from plant genomic DNA. Biotechniques 13:52–55.

    PubMed  CAS  Google Scholar 

  • Hu, J., and C.F. Quiros. 1991. Identification of broccoli and cauliflowers cultivars with RAPD markers. Plant Cell Reports. 10:505–511.

    Article  Google Scholar 

  • John, M.E., 1992. An efficient method for isolation of RNA and DNA from plants containing polyphenolics. Nucleic Acids Res. 20:2381.

    Article  PubMed  CAS  Google Scholar 

  • Klein-Lankhorst, R.M., A. Vermunt, R. Weide, T. Liharska, and P. Zabel. 1991. Isolation of molecular markers from tomato (L. esculentum) using random amplified polymorphic DNA (RAPD). Theor. Appl. Genet. 83:108–114.

    Article  CAS  Google Scholar 

  • Nelson, M. and M. McClelland. 1991. Site specific methylation: effect on DNA modification methyltransferases and restriction endonucleases. Nucleic Acids Res. 19:2045–2070.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Horticulture, Viticulture and Oenology, University of Adelaide, Waite Campus, 5064, Glen Osmond, SA, Australia

    Tina L. Maguire, Graham G. Collins & Margaret Sedgley

Authors
  1. Tina L. Maguire
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Graham G. Collins
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Margaret Sedgley
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Maguire, T.L., Collins, G.G. & Sedgley, M. A modified CTAB DNA extraction procedure for plants belonging to the family proteaceae. Plant Mol Biol Rep 12, 106–109 (1994). https://doi.org/10.1007/BF02668371

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02668371

Key Words

Search

Navigation