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Use of non-radioactive digoxigenin-labeled DNA probes for RFLP analysis in rice

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Abstract

A simple procedure for the detection of rice RFLPs with non-radioactive probes is described. Rice single-copy DNA was labeled with non-radioactive digoxigenin-d UTP. When digested total DNA was hybridized with the non-radioactive labeled DNA probes, RFLPs for rice single-copy DNA could be successfully detected.

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Abbreviations

Dig-dUTP:

digoxigenin-dUTP

RFLP:

restriction fragment length polymorphism

RG probes:

fragments from a rice genomic library

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Authors and Affiliations

  1. Plant Breeding, Genetics, and Biochemistry Division, International Rice Research Institute, P.O. Box 933, 1099, Manila, Philippines

    Takashige Ishii, Olivier Panaud, D. S. Brar & G. S. Khush

Authors
  1. Takashige Ishii
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  2. Olivier Panaud
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  3. D. S. Brar
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  4. G. S. Khush
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Ishii, T., Panaud, O., Brar, D.S. et al. Use of non-radioactive digoxigenin-labeled DNA probes for RFLP analysis in rice. Plant Mol Biol Rep 8, 167–171 (1990). https://doi.org/10.1007/BF02669512

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  • DOI: https://doi.org/10.1007/BF02669512

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