Skip to main content
SpringerLink
Log in

Non-radioactive detection of single-copy DNA-DNA hybrids

  • Protocol
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

A new procedure for non-radioactive detection of single-copy DNA-DNA hybrids combines an existing non-radioactive labeling and detection kit with a new substrate AMPPD for the enzyme alkaline phosphatase. The main advantages of this procedure are the possibility to reuse the blots easily and the much shorter detection time compared to radioactive detection methods.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Ac:

acetate

AMPPD:

3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane disodium salt

AP:

alkaline phosphatase

BCIP:

5-bromo-4-chloro-3-indolyl phosphate

anti-dig:

antibody against digoxigenin

NBT:

nitroblue tetrazolium salt

References

  • Allefs, J.J.H.M., E.M.J. Salentijn, F.A. Krens and G.J.A. Rouwendal. 1990. Optimization of non-radioactive Southern blot hybridization: single-copy detection and reuse of blots. Nucleic Acids Res. 18:3099–3100.

    Article  PubMed  CAS  Google Scholar 

  • Bronstein I. and P. McGrath. 1989. Chemiluminescence lights up. Nature 338:599–600.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre for Plant Breeding Research, CPO, Postbus 16, 6700 AA, Wageningen, The Netherlands

    C. M. Kreike, J. R. A. de Koning & F. A. Krens

Authors
  1. C. M. Kreike
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. R. A. de Koning
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. A. Krens
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kreike, C.M., de Koning, J.R.A. & Krens, F.A. Non-radioactive detection of single-copy DNA-DNA hybrids. Plant Mol Biol Rep 8, 172–179 (1990). https://doi.org/10.1007/BF02669513

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02669513

Keywords

Search

Navigation