Abstract
The Xylem Xapper is a prototype device capable of pulverizing xylem samples from stems of forest trees to a fine powder suitable for molecular biological investigations. Freezing of stem disks and pulverizing of xylem chips take place under liquid nitrogen, ensuring maximum retention and preservation of biologically active material. A modified technique enables extraction of sufficient quantities of high-quality RNA for preparation of cDNA libraries.
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Abbreviations
- LD-PCR:
-
long-distance PCR
- MDF:
-
medium-density fiberboard
- SSC:
-
sodium chloride-sodium acetate-sodium citrate
- SSTE:
-
sodium chloride-SDS-tris-EDTA
References
Beritognolo I, Magel E, Amani-Abdel A, Charpentier J-P, Jay-Allemand C, and Breton C (2002) Expression of genes encoding chalcone synthase, flavanone 3-hydroxylase and dihydroflavonol 4-reductase correlates with flavanol accumulation during heartwood formation inJunglans nigra. Tree Physiol 22: 291–300.
Chang S, Puryear J, Cairney J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11: 115–116.
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Wilson, L., Tonkin, M. & Bossinger, G. The Xylem Xapper: A device for preparation of powdered xylem from stems of forest trees for molecular biological investigations. Plant Mol Biol Rep 22, 427–434 (2004). https://doi.org/10.1007/BF02772685
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DOI: https://doi.org/10.1007/BF02772685