Abstract
Pure-grade oleuropein, a bitter, hypotensive, phenolic glucoside, was obtained from organic extracts of olive plant leaves by two Chromatographic steps. The purified compound was characterized by spectroscopic NMR and FAB-MS methods. The glucoside underwent chemical and enzymatic hydrolysis. Aglycone was characterized by spectroscopic methods (1H-NMR and FAB-MS). Glucose was measured by enzymatic methods. The enzymatic hydrolysis of oleuropein was carried out by a soluble β-glucosidase. The reaction was characterized in terms of kinetic parameters, optimal pH value, activation energy, inhibition constant by glucose, and thermal stability. Preliminary experiments were also performed in a continuousflow ultrafiltration membrane reactor. The cut-off of the membrane was lower than the molecular-weight of the enzyme, thus determining β-glucosidase confinement within the reactor. Under these conditions, β-glucosidase had a good long-term stability. This is an encouraging result in view of possible industrial applications.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Panizzi, L. M., Scarpati, M. L., and Oriente, E. G., (1960),Gazz. Chim. Ital. 90, 1449–1485.
Amiot, M. J., Fleuriet, A., and Macheix, J. J. (1989),Phytochemistry 28, 67–69 (and literature therein cited).
Ficarra, P., Ficarra, R., De Pasquale, A., Monforte, M. T., and Calabro, M. L. (1991),Il Farmaco 46, 803–815.
Kubo, I., Matsumoto, A., and Takase, I. (1985),J. Chem. Ecol. 2, 251–263.
Vaughn, R. H. (1954), inIndustrial fermentations, Underkofler, L. A. and Hickey, R. J., eds., Chemical Inc., New York,2, pp. 417–478.
Juven, B., Samish, Z., and Henis, Y. (1968),Israel J. Agr. Res. 18, 137–138.
Juven, B. and Henis, Y. (1970),J. Appl. Bacteriol. 33, 731–732.
Fleming, H. P., Walter, W. M., and Etchells, J. L. (1973),Appl. Microbiol. 26, 777–782.
Walter, W. M. Jr., Fleming, H. P., and Etchells, J. L. (1973),Appl. Microbiol. 26, 773–776.
Iocabellis, N. S., Surico, G., Evidente, A., Iasiello, I., and Randazzo, G. (1985),Phytopath. Med. 24, 315–318.
Cruess, W. V. and Alsberg, C. L. (1934),J. Amer. Chem. Soc. 56, 2115–2117.
Capasso, R., Cristinzio, G., Evidente, A., and Scognamiglio, F. (1992),Phytochemistry 12, 4125–4128.
Greco, G. Jr., Alfani, F., Iorio, G., Cantarella, M., Formisano, A., Gianfreda, L., Palescandolo, R., and Scardi, V. (1979),Biotechnol. Bioeng. 8, 1421–1438.
Breitmaier, E. and Voelter, W. (1987), inCarbon-13 NMR Spedroscopy, VCH. Verlagsgesellschaft. Weinheim. Third edition, pp. 73–84, 183–326.
Szarek, W. A., Zamotski, A., Gibson, A. R., Vyasa, D. M., and Jones, J. K. N. (1976),Can. J. Chem. 54, 3783–3793.
Iborra, J. L., Castellar, M. L., Canovas, M., and Manjon, A. (1992),Biotechnol. Lett. 6, 475–480.
Lo Scalzo, R. and Scarpati, M. L. (1993),J. Nat. Prod. 56, 621–623.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Capasso, R., Evidente, A., Visca, C. et al. Production of glucose and bioactive aglycone by chemical and enzymatic hydrolysis of purified oleuropein fromOlea Europea . Appl Biochem Biotechnol 61, 365–377 (1997). https://doi.org/10.1007/BF02787808
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02787808