Abstract
A new strain ofBacillus subtilis C 126 was isolated from sugar cane fermentation and produced an antibiotic that inhibited the growth ofMicrococcus flavus. The production of the antibiotic in culture medium followed to extraction withn-butanol, thin layer chromatography, and microbiological tests indicated that a polypeptide antibiotic was produced. The fraction obtained by Sephadex G-25 column and analyzed by HPLC indicated that bacitracin complex was produced.
Similar content being viewed by others
References
Anker, H. S., Johnson, B. A., Goldenberg, J., and Meleney, F. L. (1948),J. Bacteriol.55, 249–255.
Hickey, R. J. (1964),Industrial Microbiology, vol.5, D. J. D. Hockenhull, ed., Temple Pres, London, pp. 93–150.
Weinburg, E. D. (1968),Antibiotics: Mechanism of Action, vol.1, Springer-Verlag, New York, pp. 90–101.
Bodanszky, M. and Perlman, D. (1969),Science 163, 352–358.
Haavik, H. I. (1974),J. Gen. Microbiol. 81, 383–390.
Jarvis, F. G. and Johnson, M. J. (1947),Am. Chem. Soc. 69, 3010–3017.
Winnick, R. E., Lis, H., and Winnick, T. (1961),Biochim. Biophys. Acta 49, 451–462.
Demain, A. L. (1963),Clin. Med. 70, 2045–2051.
Mach, B., Reich, E., and Tatum, E. L. (1963),Froc. Nat. Acad. Sci. USA 50, 175–181.
Katz, E., Wise, M., and Weissbach, H. (1965),J. Biol. Chem. 240, 3071–3078.
Banerjee, A. B., Majundar, S. K., and Bose (1967),Antibiotics, vol.2, D. Gottlieb and P. D. Show, eds., Springer-Verlag, NY, pp. 271–275.
Bernlohr, R. W. and Novelli, G. D. (1960),Arch. Biochem. Biophys. 87, 232–238.
Snoke, J. E. (1960),J. Bacteriol. 80, 552–557.
Snoke, J. E. (1961),J. Bacteriol. 81(06), 986–989.
Haavik, H. I. (1974),J. Gen. Microbiol. 84, 226–230.
Haavik, H. I. and Thomassen, S. (1973),J. Gen. Microbiol. 76, 451–454.
Rabinovitch, L. (1970),Rev. Bras. Farm. 51, 205–208.
Grove, D. C. and Randall, W. (1955), Medical Encyclopedia, New York, pp. 77–81.
Handlin, D. (1949),Arch. Biochem. 24, 435–446.
Lowry, O. H., Rose Brough, N. J., Farr, A. L., and Randall, R. J. (1951),J. Biol. Chem. 193, 265–275.
Johnson, B. A., Anker, H., and Meleney, F. L. (1945),Science 102, 376–377.
Gelotte, B. (1960),J. Chromatog. 3, 330–342.
Tsuji, K., Robertson, J. H., and Bach, J. A. (1974),J. Chromatog. 112, 633–672.
Tsuji, K. (1975),J. Chromatog. 112, 633–672.
Demain, A. L. (1978),Lloydia 31, 395–418.
Bernlohr, R. W. and Novelli, G. D. (1963),Arch. Biochem. Biophys. 103, 94–104.
Haavik, H. I. (1974),J. Gen. Microbiol. 84, 321–326.
Rabinovitch, L. (1971),Mem. Inst. Oswaldo Cruz 69, 121–130.
Rabinovitch, L. and Filho, M. V. S. (1982),Rev. Microbiol. 13(3), 215–224.
Haavik, H. I. (1976),J. Gen. Microbiol. 96, 393–399.
Haavik, H. I. and Froyshov, O. (1975),Nature 254, 78–82.
Tsuji, K., Robertson, J. H., and Bach, J. A. (1975),J. Chromatog.112, 633–672.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Azevedo, E.C., Rios, E.M., Fukushima, K. et al. Bacitracin production by a new strain ofBacillus subtilis . Appl Biochem Biotechnol 42, 1–7 (1993). https://doi.org/10.1007/BF02788897
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02788897