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Interactive metabolic regulations during biomethanation ofLeucaena leucocephala

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Abstract

Biomethanation of leaves of the legumeL. leucocephala operated in batch reactors at different input volatile solids (12–18 g/L) proceeded in distinct metabolic phases. An initial cellulolytic phase of 4 d was followed by an early and active methanogenic phases (5–21 d) and a terminal phase of low-rate methanogenesis. Hydrolysis of cellulose was concentration-dependent and resulted in increased volatile acid levels. The trend of changes showed some variations at different input volatile solids. The changes in the levels of volatile acids followed an oscillatory pattern. The controlled rate of cellulose hydrolysis, levels of volatile acids, and steady-state levels of soluble carbohydrates and reducing sugars observed during active methanogenesis are indicative of interactive metabolic regulations.

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References

  1. Zeikus, J. G. (1980),Ann. Rev. Microbiol. 34, 423.

    Article  CAS  Google Scholar 

  2. Baresi, L., Mah, R. A., Ward, D. and Kaplan, I. R. (1978),Appl. Env. Microbiol. 36, 186.

    CAS  Google Scholar 

  3. Mah, R. A., Smith, M. R.. and Baresi, L. (1978),Appl. Env. Microbiol. 35, 1174.

    CAS  Google Scholar 

  4. White, L. P. and Plaskett, L. G. (1981),Biomass as Fuel, Academic, New York.

    Google Scholar 

  5. Bungay, H. R. (1983),Environ. Sci. Technol. 17, 24A.

    Article  CAS  Google Scholar 

  6. Hashimoto, A. G., Varel, V. H., and Chen, Y. R. (1980),Ind. Eng. Chem. Pro. Res. Dev. 19, 471.

    Article  Google Scholar 

  7. Naveau, H., Nyns, E. J., and Pauss, A. (1984),Anaerobic Digestion and Carbohydrate Hydrolysis of Waste, Ferrero, G., Naveau, H., and Ferranti, M. P., eds., Elsevier Applied Science Publisher, London, p. 209.

    Google Scholar 

  8. Chen, Y. R. and Hashimoto, A. G. (1978),Biotech. Bioeng. Symp. 8, 289.

    Google Scholar 

  9. Narayanaswamy, V., Sankar, K., Chandrasekaran, P. M., and Lalitha, K. (1986),Fuel 65, 1129.

    Article  Google Scholar 

  10. Chynoweth, D. P., Ghosh, S., and Klass, D. L. (1981),Biomass Conversion Processes for Energy and Fuels, Sofer, S. S. and Zaborsky, O. R., eds., Plenum, New York, p. 315.

    Google Scholar 

  11. Weimer, P. J. and Zeikus, J. G. (1979),Appl. Env. Microbiol. 33, 289.

    Google Scholar 

  12. Winter, J. and Wolfe, R. S. (1979),Arch. Microbiol. 121, 97.

    Article  CAS  Google Scholar 

  13. Laube, V. M. and Martin, S. M. (1981),Appl. Env. Microbiol. 42, 413.

    CAS  Google Scholar 

  14. Khan, A. W. (1977),Can. J. Microbiol. 23, 1700.

    Article  CAS  Google Scholar 

  15. David B. Archer (1984), Biochem. Soc. Transcations, London.

  16. Standard Methods for the Examination of Water and Waste Water, 14th edition, American Public Health Association, New York (1975).

  17. Updegraff, D. M. (1969),J. Biol. Chem. 32, 420.

    CAS  Google Scholar 

  18. Dubois, M., Giller, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956),Anal. Chem. 28, 350.

    Article  CAS  Google Scholar 

  19. Miller, G. L. (1959),Anal. Chem. 31, 426. Meeting. 12, 1144.

    Article  CAS  Google Scholar 

  20. Giuliano, C. and Khan, A. W. (1985),Biotech. Bioeng. 26, 980.

    Article  Google Scholar 

  21. Koster, I. W. and Cramer, A. (1987),Appl. Env. Microbiol. 53, 403.

    CAS  Google Scholar 

  22. Chandler, J. A., Jewell, W. J., Gossett, J. M., van Soest, P. J., and Robertson, J. B. (1980),Biotech. Bioeng. Symp. 10, 93.

    CAS  Google Scholar 

  23. Zeikus, J. G. (1977),Bacteriol. Rev. 41, 514.

    CAS  Google Scholar 

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Krishnan, S., Lalitha, K. Interactive metabolic regulations during biomethanation ofLeucaena leucocephala . Appl Biochem Biotechnol 26, 73–84 (1990). https://doi.org/10.1007/BF02798394

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  • DOI: https://doi.org/10.1007/BF02798394

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