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Immobilization of a photosystem ii submembrane fraction in a glutaraldehyde cross-linked matrix

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Abstract

A wide range of immobilization procedures have been shown to stabilize the functions of photosynthetic materials. The purpose of this work was to determine if the above procedures can be applied to submembrane fractions. Triton X-100-derived photosystem II submembrane fractions isolated from spinach were immobilized in a glutaraldehyde cross-linked albumin matrix. The optimal conditions were obtained in presence of 1 mM NaCl and 5 mM MgCl2. The treated membranes were less affected by long-term storage at 4°C, high pH and temperature, and strong light exposure. The results are discussed in terms of a diffusion barrier resulting from the immobilization matrix.

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Authors and Affiliations

  1. Centre de Recherche en Photobiophysique, Université du Québec à Trois-Rivières, C.P. 500, G9A 5H7, Trois-Rivières, Québec, Canada

    Robert Carpenter & Sylvie Lemieux

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  1. Robert Carpenter
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  2. Sylvie Lemieux
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Carpenter, R., Lemieux, S. Immobilization of a photosystem ii submembrane fraction in a glutaraldehyde cross-linked matrix. Appl Biochem Biotechnol 15, 107–117 (1987). https://doi.org/10.1007/BF02801312

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  • DOI: https://doi.org/10.1007/BF02801312

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