Abstract
A set of vectors has been developed that simplify shuttling expression cassettes between small plasmids of high copy number ideal for experiments involving biolistic transient expression and a binary transformation plasmid. Three cassettes for the expression of a cloned coding sequence behind different promoters have been modified; combinations of these cassettes can be excised withNot I, and sequentially cloned into the transformation vector in a procedure that removes the first cloning site. The system is demonstrated by inducing anthocyanin synthesis with paired regulatory genes of maize biolistically delivered to a maize cell suspension, and then expressed in transformed tobacco.
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Abbreviations
- RbcS :
-
plant-wide family of genes encoding ribulose bisphosphate carboxylase
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de Majnik, J., Joseph, R.G., Tanner, G.J. et al. A convenient set of vectors for expression of multiple gene combinations in plants. Plant Mol Biol Rep 15, 134–140 (1997). https://doi.org/10.1007/BF02812263
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DOI: https://doi.org/10.1007/BF02812263