Skip to main content
Log in

Lipase production byAspergillus andPenicillium species

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Forty each of aspergilli and penicillia were screened for extracellular lipase production on agar plates and in liquid medium containing olive oil as substrate. Twenty-nine aspergilli and twenty-six penicillia produced lipase. Out of these, 19 aspergilli and 22 penicillia showed activity both on Nile blue sulfate and glycerol tributyrate agar plates while only 10 aspergilli and 4 penicillia showed a positive response to glycerol tributyrate agar alone. The screening revealed 11Aspergillus spp. and 15Penicillium spp. as new lipase producers. Pig fat as an economic substrate for lipase production was also investigated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Abbadi N.E., Druet D., Comeau L.C.: Immunocytochemical identification and localization of lipase in cells of the mycelium ofPenicillium cyclopium variety.Appl. Microbiol. Biotechnol. 42, 923–930 (1995).

    Article  CAS  Google Scholar 

  • Alford J.A., Steinle E.E.: A double layered plate method for the detection of microbial lipolysis.J. Appl. Bact. 30, 488–494 (1967).

    CAS  Google Scholar 

  • Bhaduria R.: Lipolytic micro-organisms isolated from dry fruits.Sci. Culture 55, 173–175 (1989).

    Google Scholar 

  • Bjorkling F., Godtfredsen S.E., Kirk O.: The future impact of industrial lipases.Trends Biotechnol. 9, 360–363 (1991).

    Article  Google Scholar 

  • Chander H., Batish V.K., Sannabhadti S.S., Srinivasan R.A.: Factors affecting lipase production inAspergillus wentii.J. Food Sci. 45, 598–600 (1980).

    Article  CAS  Google Scholar 

  • Eitenmiller R.R., Vakil J.R., Shahani K.M.: Production and properties ofPenicillium roqueforti lipase.J. Food. Sci. 35, 130–133 (1970).

    Article  CAS  Google Scholar 

  • Ghosh P.K., Saxena R.K., Gupta R., Yadav R.P., Davidson S.: Microbial lipases: production and applications.Sci. Prog. 79, 119–157 (1996).

    PubMed  CAS  Google Scholar 

  • Hou C.T., Johnston T.M.: Screening of lipase activities with cultures from theAgricultural Research Service Culture Collection.J. Am. Oil Chem. Soc. 69, 1090–1097 (1992).

    Article  Google Scholar 

  • Karnetová J., Matějů J., Řezanka T., Procházka P., Nohýnek M., Rokos J.: Estimation of lipase activity by the diffusion plate method.Folia Microbiol. 29, 346–347 (1984).

    Google Scholar 

  • Kokusho Y., Machida H., Iwasaki S.: Studies on alkaline lipase production: isolation and identification of lipase producing microorganisms.Agric. Biol. Chem. 46, 1159–1164 (1982).

    CAS  Google Scholar 

  • Kouker G., Jaeger K.E.: Specific and sensitive plate assay for bacterial lipases.Appl. Environ. Microbiol. 53, 211–213 (1987).

    PubMed  CAS  Google Scholar 

  • Lawrence R.C., Fryer T.F., Reiter B.: Rapid method for the quantitative estimation of microbial lipases.Nature 213, 1264–1265 (1967).

    Article  CAS  Google Scholar 

  • Macrae A.R., Hammond R.C.: Present and future applications of lipases.Biotechnol. Genet. Eng. Rev. 3, 193–217 (1985).

    CAS  Google Scholar 

  • Macris J.B., Kourentzi E., Hatzinikolaou D.G.: Studies on localization and regulation of lipase production byAspergillus niger.Process Biochem. 31, 807–812 (1996).

    Article  CAS  Google Scholar 

  • Nahas E.: Control of lipase production byRhizopus oligosporus under various growth conditions.J. Gen. Microbiol. 134, 227–233 (1988).

    CAS  Google Scholar 

  • Parmar V.S., Pati H.N., Yadav R.P., Kumar A., Bisht K.S., Gupta R., Davidson S., Poonam, Saxena R.K.: Utility of a novel lipase fromAspergillus terreus in deacetylation reactions.Biocatal. Biotransform. 16, 17–25 (1998).

    CAS  Google Scholar 

  • Pimentel M.C.B., Melo E.H.M., Filho J.L.L., Duran N.: Production of lipase free of citrinin byPenicillium citrinum.Mycopathologia 133, 119–121 (1996).

    Article  CAS  Google Scholar 

  • Saxena R.K.: Studies on the control of reproductive differentiation inAspergillus nidulans under submerged condition.PhD Thesis. University of Delhi (India) 1976.

    Google Scholar 

  • Tan K.H., Gill C.O.: Effect of culture conditions on batch growth ofSaccharomycopsis lipolytica on olive oil.Appl. Microbiol. Biotechnol. 20, 201–206 (1984).

    Article  CAS  Google Scholar 

  • Tan K.H., Gill C.O.: Effect of culture conditions on batch growth ofPseudomonas fluorescens on olive oil.Appl. Microbiol. Biotechnol. 23, 27–32 (1985).

    Article  CAS  Google Scholar 

  • Vulfson E.N.: Industrial applications of lipases, pp. 271–288 in P. Woolley, S.B. Petersen (Eds):Lipases, Their Structure, Biochemistry and Applications, Cambridge University Press, Great Britain 1994.

    Google Scholar 

  • Yadav R.P.: Production, purification and characterization of an industrially important lipase fromAspergillus terreus.PhD Thesis. University of Delhi (India) 1995.

    Google Scholar 

  • Yadav R.P., Saxena R.K., Gupta R., Davidson S.: Production of biosurfactant from sugar alcohols and natural triglycerides byAspergillus terreus lipase.J. Sci. Industr. Res. 56, 479–482 (1997).

    CAS  Google Scholar 

  • Yadav R.P., Saxena R.K., Gupta R., Davidson S.: Purification and characterization of a regiospecific lipase fromAspergillus terreus. Biotechnol. Appl. Biochem., in press (1998a).

  • Yadav R.P., Saxena R.K., Gupta R., Davidson S. Rapid zymogram for lipase.Biotechniques 24, in press (1998b).

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yadav, R.P., Saxena, R.K., Gupta, R. et al. Lipase production byAspergillus andPenicillium species. Folia Microbiol 43, 373–378 (1998). https://doi.org/10.1007/BF02818576

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02818576

Keywords

Navigation