Skip to main content
SpringerLink
Log in

Potato variety identification by use of electrophoretic patterns of tuber proteins and enzymes

  • Published:
American Potato Journal Aims and scope Submit manuscript

Summary

Forty-five potato varieties were analysed for three biochemical systems obtained from tuber extracts. The systems employed were esterase and peroxidase isozymes and soluble-proteins patterns; separation was accomplished by disc electrophoresis. Five esterase isozymes, a group of eight peroxidase isozymes and more than 20 proteins were present in unique combinations specific for each variety. This is an effective and efficient procedure for variety identification.

Resumen

Extractos de tubérculos de cuarenta y cinco variedades de papa fueron analizados en tres diferentes sistemas bioquimicos. Los sistemas empleados fueron : las isozimas, —esterasa y peroxidasa y las proteínas solubles separadas por electroforesis de disco. Se encontraron cinco esterasas; un grupo de ocho peroxidasas y más de veinte proteínas presentes en una combinatión única y específica para cada variedad. Este es un procedimiento efectivo y eficiente para la identification de variedades.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Desborough, S. and S. J. Peloquin. 1966. Disc electrophoresis of tuber proteins fromSolanum species and interspecific hybrids. Phytochem. 5: 727–733.

    Article  CAS  Google Scholar 

  2. Desborough, S. and S. J. Peloquin, 1967. Esterase isozymes fromSolanum tubers. Phytochem. 6: 989–994.

    Article  CAS  Google Scholar 

  3. Fox, D. J., D. A. Thutnan and D. Boulter. 1964. Studies on the proteins of seeds of the Leguminoseae. I. Albumins. Phytochem. 3: 417–419.

    Article  CAS  Google Scholar 

  4. Frankel, T. N. and E. D. Garber. 1965. Esterases in extracts from germinating seeds of twelve pea varieties. Botan. Gaz. 126: 221–222.

    Article  CAS  Google Scholar 

  5. Garber, E. D. 1965. The genusCollinsia XXVIII. A paper Chromatographic and disc electrophoresis study of leaf extracts from 14 species and progeny from 5 interspecific hybrids. Can. J. Genet. Cytol. 7: 551–558.

    CAS  Google Scholar 

  6. Gell, P. G. H., J. G. Hawkes and S. T. C. Wright. 1960. The application of immunological methods to the taxonomy of species within the genusSolanum. Proc. Roy. Soc. B 151: 364–383.

    Article  CAS  Google Scholar 

  7. Hart, G. E. and C. R. Bhatia. 1967. Acrylamide gel electrophoresis of soluble leaf proteins and enzymes fromNicotiana species. Can. J. Genet. Cytol. 9: 367–374.

    CAS  Google Scholar 

  8. Hawkes, J. G. and R. N. Lester. 1966. Immunological studies of the tuber-bearing solanums. II. Relationships of the North American species. Ann. Bot. 30: 269–290.

    Google Scholar 

  9. Johnson, B. L. 1967. Tetraploid wheats: seed protein electrophoretic patterns of the Emmer and Timopheeri Groups. Sci. 158: 131–132.

    Article  CAS  Google Scholar 

  10. Loeschcke, V. and H. Stegemann. 1966. Proteine der kartoffel-knollen in abhängigkeit von sorte und virosen (polyacrylamidelektrophorese). Phytochem. 5: 985–991.

    Article  CAS  Google Scholar 

  11. Law, R. B. and I. G. Wool. 1967. Mammalian ribosomal protein-analysis by electrophoresis of polyacrylamide gel. Sci. 155:330.

    Article  Google Scholar 

  12. Mecklenburg, Helen C. 1966. Inflorescence hydrocarbons of some species ofSolanum L. and their possible taxonomic significance. Phytochem. 5: 1201–1210.

    Article  CAS  Google Scholar 

  13. Scandalios, J. G. 1965. Subunit dissociation and recombination of catalase isozymes. P.M.A.S. 53: 1035–1040.

    Article  CAS  Google Scholar 

  14. Schwartz, D. 1960. Genetic studies on mutant enzymes in maize: synthesis of hybrid enzymes by heterozygotes. P.M.A.S. 46: 1210–1215.

    Article  CAS  Google Scholar 

  15. Vaughan, J. G. and A. Waite. 1967. Comparative electrophoresis studies of the seed proteins of certain species ofBrassica andSinapis. J. Exp. Bot. 18: 100–109.

    Article  CAS  Google Scholar 

  16. West, N. B. and E. D. Garber. 1967. Genetic studies of variant enzymes. I. An electrophoretic survey of esterases and leucine aminopeptidase in the genusPhaseolus. Can. J. Genet. Cytol. 9: 640–645.

    CAS  Google Scholar 

  17. Zwartz, Judith A. 1966. Potato varieties and their protein electropherogram characteristics. Eur. Potato J. 9: 111–128.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Horticulture and Genetics, University of Wisconsin, Madison, Wisconsin

    Sharon Desborough & S. J. Peloquin

Authors
  1. Sharon Desborough
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. J. Peloquin
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Paper No. 1167 from the laboratory of Genetics, University of Wisconsin. Supported in parts by grants from the National Science Foundation and the Rockefeller Foundation. SD was a National Institute of Health Predoctoral Trainee (GM-398).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Desborough, S., Peloquin, S.J. Potato variety identification by use of electrophoretic patterns of tuber proteins and enzymes. American Potato Journal 45, 220–229 (1968). https://doi.org/10.1007/BF02849396

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02849396

Keywords

Search

Navigation