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Effect of scoparone on the hepatic microsomal UDP glucuronyltransferase activity in mice

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Abstract

The effect of scoparone on UDPglucuronyltransferase in mouse hepatic microsomes was studied. After treatment with scoparone, hepatic microsomal UDPglucuronyltransferase activity was increased with dose-dependent manner as compared to control. The Vmax value (control=23.2 n moles/mg protein/min, scoparone=31.2 n moles/ mg protein/min) without affecting the Km value (414 μM) for p-nitrophenol was increased by the scoparone treatment, and the pattern of kinetic studies for UDP-glucuronic acid was also similar to those of p-nitrophenol. Whereas, the hepatic microsomal UDPglucoronyltransferase was not changed by the addition of scoparone in vitro. The results obtained suggest that the characteristics of increase in the enzyme activity may include induction of enzyme proteins.

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  1. Department of Pharmacology, College of Pharmacy, Yeungnam University, 632, Gyongsan, Korea

    Keun Huh, Sang-Il Lee & Jong-Min Park

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  1. Keun Huh
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  2. Sang-Il Lee
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Huh, K., Lee, SI. & Park, JM. Effect of scoparone on the hepatic microsomal UDP glucuronyltransferase activity in mice. Arch. Pharm. Res. 10, 165–168 (1987). https://doi.org/10.1007/BF02861907

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