Abstract
DNA synthesis after the ultraviolet irradiation was followed in the excision proficient strainEscherichia coli B/rHcr +, in which the ability to excise thymin dimers was suppressed by a preirradiation inhibition of DNA and protein syntheses and in the excision deficient strainEscherichia coli B/rHcr −. Synthesis of pulse-labeled DNA, its stability and semiconservative DNA synthesis were compared in both strains. It was found that cells of theHcr + strain restore semiconservative DNA synthesis and the pulselabeled DNA appears stable, in spite of the fact that dimers are not excised under these conditions. On the other hand, cells of theHcr − strain are unable to restore semiconservative DNA synthesis and the pulselabeled DNA is degraded. As the repair by the excision of dimers under the used experimental conditions may be excluded in both strains, it is possible to assume that activity of enzymes included in theHcr + marker is prerequisite for restoring the DNA synthesizing system in theHcr + strain.
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Brozmanová, J., Slezáriková, V. & Sedliaková, M. DNA synthesis in pretreated and ultraviolet-irradiated cells ofEscherichia coli B/rHcr + andHcr − . Folia Microbiol 19, 459–465 (1974). https://doi.org/10.1007/BF02872910
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DOI: https://doi.org/10.1007/BF02872910