biologia plantarum

Biologia plantarum 38:191, 1996 | DOI: 10.1007/BF02873845

Effect of wounding on nucleotide pools inBidens pilosa L.

C. Henry-Vian¹, A. Vian¹, G. Ledoigt¹, M. -O. Desbiez¹

¹ Unités associées INRA, Université Blaise Pascal, Clermont-Ferrand, France

Wounding both cotyledons ofBidens pilosa (var.radiatus) induces the inhibition of hypocotyl growth. The wound signal is transmitted very rapidly from cotyledon to hypocotyl and can be visualized by the change in nucleotide pools. First we have shown that the irradiance of the plant can change the ATP level without plant wounding. Therefore, plants were harvested at the start of the light period. Under these conditions, we have determined in hypocotyl the levels of adenosine triphosphate (ATP), guanosine triphosphate (GTP) and non adenylic triphosphates (NTP), and adenylate energy charge (AEC) after wounding. We have observed a transient (2 min) increase in the ATP level followed by a decrease 5 to 30 min later. A similar result was obtained for the GTP level but with some delay. The GTP level increased in 5 min and then decreased after 60 min. For the NTP level the decrease is effective from 5 to 60 min after wounding. The calculation of AEC has shown that a very tight control in the level of ATP may be involved in response to wounding.

Keywords: ATP; distant response; signal transduction

Received: July 18, 1995; Accepted: November 9, 1995; Published: June 1, 1996  Show citation