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In vitro photoinduction of leaf tissue ofStreptocarpus nobilis

  • Original Papers
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Biologia Plantarum

Abstract

Leaf expiants from vegetative plants of the short-day plantStreptocarpus nobilis (C. B. Clarke) developed flower budsin vitro when cultured in 8 h photoperiods. Tn non-inductive photoperiods only vegetative buds were formed.In vitro photoinduction was demonstrated by giving the expiants short-day (SD) cycles and then transferring them to non-inductive photoperiods for expression of flowering. On medium containing 6-benzylaminopurine (BAP) organogenesis was initiated during the photoinductive treatments. Photoinduction of leaf tissue without adventitious bud development was obtained on medium without BAP. The photoinductive state of the leaf tissue was fairly stable, being expressed after 2–3 weeks in non-inductive photoperiods when adventitious buds were formed. The quantitativein vitro flowering response to the endogenous floral stimuli, resulting from photoinduction, could provide the basis of a bioassay for presumptive flower inducing chemicals.

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  1. Research Station, Agriculture Canada, K1A OC6, Ottawa, Canada

    J. Simmonds

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Simmonds, J. In vitro photoinduction of leaf tissue ofStreptocarpus nobilis . Biol Plant 27, 318–324 (1985). https://doi.org/10.1007/BF02879869

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