Skip to main content
Log in

The chitin-glucan complex inSaccharomyces cerevisiae

I. IR and X-ray observations

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

The content of glucosamine in the walls of daughter (without bud scars) and mother (multiscar) cells ofSaccharomyces cerevisiae was examined in a control and after treatment with dilute alkali, acid and buffer. The occurrence of chitin in the bud and birth scars is discussed. The results of IR and X-ray analysis of cell-wall fractions indicate the presence of α-chitin which is a part of the chitin-glucan complex. The size of the crystallite of α-chitin in this complex is about 60 Å.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bacon J. S. D., Davidson E. D., Taylor I. F.: The location of chitin in the yeast cell wall.Biochem. J.101, 36 C (1966).

    Google Scholar 

  • Barker S. A., Bourne E. J., Whiffen D. H.: Use of infrared analysis in the determination of carbohydrate structure, p. 213, in D. Glick (Ed.):Methods of Biochemical Analysis, Vol. III (Interscience Publishers: New York) 1956.

    Chapter  Google Scholar 

  • Beran K.: At the IIIrd Symposium on yeast. Delft-Hague 1969. Abstr. No. B3

  • Beran K.: At the IInd International Symposium on Yeast Protoplasts. Brno 1968. Published inActa Facultatis Medicae Universitatis Brunensis37, (1970).

  • Carlström D.: The crystal structure of α-chitin (Poly-N-Acetyl-d-glucosamine).J. Biophys. Biochem. Cytol.3, 669 (1957).

    Article  PubMed  Google Scholar 

  • Carlström D.: The polysaccharide chain of chitin.Biochim. Biophys. Acta59, 361 (1962).

    Article  PubMed  Google Scholar 

  • Dweltz N. E.: The structure of chitin.Biochim. Biophys. Acta.44, 416 (1960).

    Article  PubMed  CAS  Google Scholar 

  • Gatt R., Berman E. R.: A rapid procedure for the estimation of aminosugars on a microscale.Anal. Biochem.15, 167 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Hackman R. H., Goldberg M.: Studies on chitin. VI The nature of α- and β-chitins.Aust. J. Biol. Sci.18, 935 (1965).

    PubMed  CAS  Google Scholar 

  • Holan Z., Beran K., Procházková V., Baldrián J.: Gas liquid chromatographic determination of the content of N-acetylglucosamine and its polymers in the cell wall of yeast. 1st Specialized International Symposium on Yeasts.Yeast as Models in Science and Technics, Smolenice 1971.

  • Houwink A. L., Kreger D. R.: Observation on the cell wall of yeasts. An electron microscope and X-ray diffraction study.Antonie van Leeuwenhoek,19, 1 (1953).

    Article  PubMed  CAS  Google Scholar 

  • Jones G. H., Ballou C. E.: Studies on the structure of yeast mannan. I. Purification and some properties of an α-mannosidase from anArthrobacter species.J. Biol. Chem.244, 1043 (1969a).

    PubMed  CAS  Google Scholar 

  • Jones G. H., Ballou C. E.: Studies on the structure of yeast mannan. II. Mode of action of theArthrobacter α-mannosidase on yeast mannan.J. Biol. Chem.244, 1052 (1969b).

    PubMed  CAS  Google Scholar 

  • Kessler G., Nickerson W. J.: Glucomannan complexes from cell walls of yeast.J. Biol. Chem.234, 2281 (1959).

    PubMed  CAS  Google Scholar 

  • Klug H. P., Alexander L. E.: X-Ray diffraction procedures. J. Willey & Sons, Chapter 9, New York 1954.

    Google Scholar 

  • Kreger D. R., Meeuse B. J. D.: X-Ray diagrams ofEuglena-paramylon, of the acid-insoluble glucan of yeast cell walls and of laminarin.Biochim. Biophys. Acta.9, 699 (1952).

    PubMed  CAS  Google Scholar 

  • Kreger D. H.: Observations on cell walls of yeasts and some other fungi by X-ray diffraction and solubility test.Biochim. Biophys. Acta13, 1 (1954).

    Article  PubMed  CAS  Google Scholar 

  • Lieblová J., Beran K.: Synchronous and synchronized culture ofSaccharomyces cerevisiae with respect to relative age of individual cells,Folia Microbiol.16, 241 (1971).

    Article  Google Scholar 

  • Marx-Figini M., Schulz G. V.: Über die Kinetik und den Mechanismus der Biosynthese der Cellulose in den höheren Pflanzen (nach Versuchen an den Samenhaaren der Baumwolle).Biochim. Biophys Acta112, 81 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Michell A. J., Scurfield G.: Composition of extracted fungal cell walls as indicated by infrared spectroscopy.Arch. Biochem. Biophys120, 628 (1967).

    Article  CAS  Google Scholar 

  • Michell A. J., Scurfield G.: An assessment of infrared spectra as indicators of fungal cell wall composition.Aust. J. Biol. Sci.23, 345 (1970).

    CAS  Google Scholar 

  • Misaki A. Johnson J., Kirkwood S., Scaletti J. V., Smith F.: Structure of the cell wall glucan of yeast (Saccharomyces cerevisiae)Carbohyd. Res.6, 150 (1968).

    Article  CAS  Google Scholar 

  • Nečas O.: Cell wall synthesis in yeast protoplasts.Bact. Rev.,35, 149 (1971).

    PubMed  Google Scholar 

  • Neely W. B.: Infrared spectra of carbohydrates.Adv. Carbohyd. Chem.12, 13 (1957).

    CAS  Google Scholar 

  • Northcote D. H., Horne R. W.: The chemical composition and structure of the yeast cell wall.Biochem. J.51, 233 (1952).

    Google Scholar 

  • Olson B., Johnson M. J.: Factors producing high yeast yield in synthetic media.J. Bacteriol.57, 235 (1949).

    CAS  Google Scholar 

  • Rees D. A., Scott W. E.: Polysaccharide conformation. VI. Computer model-building for linear and branched pyranoglycans. Correlations with biological function. Preliminary assessment of inter-residue forces in aqueous solution. Further interpretation of optical rotation in terms of chains conformation.J. Chem. Soc. (B) 469 (1971).

  • Rinaudo M., Barnoud F., Merle J. P.: Enzymatic degradation of cellulosic fibers.J. Polymer Sci. (C)28, 197 (1969).

    Google Scholar 

  • Rudall K. M.: The chitin/protein complexes of insect cuticles.Adv. Insect Physiol.1, 257 (1963).

    Article  CAS  Google Scholar 

  • Spedding H.: Infrared spectroscopy and carbohydrate chemistry.Adv. Carbohyd. Chem.19, 23 (1964).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Beran, K., Holan, Z. & Baldrián, J. The chitin-glucan complex inSaccharomyces cerevisiae . Folia Microbiol 17, 322–330 (1972). https://doi.org/10.1007/BF02884098

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02884098

Keywords

Navigation