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The content of ATP, ADP, AMP, Pi, the activity of enzymes involved in the glycolytic pathway and some problems of its regulation, and energy balance in tobacco plants infected with potato virus Y

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Biologia Plantarum

Abstract

The content of ATP, ADP, AMP, Pi, the activity of the enzymes involved in the glycolytic pathway, some problems of their regulation by adenine nucleotides and some basic problems connected with tissue energy balance were studied in tobacco plants infected with the potato virus Y (PVY). The contents of ATP and ΣAdN were increased in virus-infected tissues when compared with healthy tissues and correlated with the PVY reproduction curve. ADP and AMP contents decreased just after the inoculation and increased at the end of the experimental period, Pi content was not influenced by the infection.

The activities of the key enzymes of the glycolytic pathway (6-phosphofructokinase, hexosediphosphatase, and pyruvate kinase), determined both in crude homogenates and after partial purification, did not differ during the entire experimental period from the values found in healthy control tissues, similarly as the activities of glucosephosphate isomerase, glyceraldehydephosphate dehydrogenase, phosphoglyceromutase and enolase observed in crude homogenates. The unchanging AEC value in virus-infected tissues simultaneously indicated that no change in the rate of the glycolytic pathway occurred even under “invivo” conditions at the period of the acute stage of infection.

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  1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Na Karlovce 1, 160 00, Praha 6, Czechoslovakia

    L. Sindelár

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Sindelár, L. The content of ATP, ADP, AMP, Pi, the activity of enzymes involved in the glycolytic pathway and some problems of its regulation, and energy balance in tobacco plants infected with potato virus Y. Biol Plant 28, 449–459 (1986). https://doi.org/10.1007/BF02885049

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  • DOI: https://doi.org/10.1007/BF02885049

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