Abstract
N-malonyl-D-tryptophan (MT) and D-tryptophan added to the medium instead of auxin stimulated growth of soybean and tomato cell and tissue cultures. Effects of 50–100 μmol 1-1 MT and 100 –300 μmol 1-1 D-tryptophan were equal to the effect of 3–10 μmol 1-1 IAA. Soybean cells grown in the presence of 100 μmol 1-1 MT contained 125–170 ng IAA per 1 g fresh mass (as determined by spectrofluorimetric indole-α-pyrone method), whereas the cells grown in the presence of NAA 10. 7 μmol 1-1 contained 50 –60 ng IAA and the cells grown in the absence of auxin failed to show endogenous IAA. MT as proposed can be hydrolyzed by plant cells with liberation of D-tryptophan, which in turn can be used in IAA synthesis. It is proposed that MT is a possible source of endogenous auxin in plants.
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Abbreviations
- MT:
-
N-malonyl-D-tryptophan
- IAA:
-
indol-3-yIacetic acid
- NAA:
-
1-naphthylacotic acid
- D-trp:
-
D-tryptophan
References
Elliott, M. C.: α-N-malony]-D-tryptophan in seedling wheat roots. -New Phytol.70: 1005 to 1015, 1971.
Gamburg, K. Z., Osharova, L. M.: [The action of auxin on the mitotic cycle of tobacco cells growing in suspension culture.] In Russ. -Tsitologiya16: 681–689, 1973.
Good, N. E., Andreae, W. A.: Malonyltryptophan in higher plants. -Plant Physiol.32: 561 to 565, 1957.
Kutáček, M., Kefeli, V.: Biogenesis of indole compounds from D- and L-tryptophan in segments of etiolated seedlings of cabbage, maize and pea. -Biol. Plant.12: 145–158, 1970.
Rekoslavskaya, N. I., Gamburg, K. Z.: On the biological activity of IAA conjugates. -Biochem. Physiol. Pflanzen169: 299–303, 1976.
Rekoslavskaya, N. I., Gamburg, K. Z.:Do tomato plants contain endogenous indoleacetylaspar-tic acid? -Biol. Plant.25: 166–172, 1983.
Rekoslavskaya, N. I., Gamburg, K. Z.: [The induction of MT formation in plants by water deficit.] In Russ.-Fiziol. Rast.32: 193–196, 1985.
Sandberg, G., Sandberg, A.: Precision and accuracy of indole-3-acetic acid analysis performed with the 2-methyl-indolo-α-pyrone fluorescence assay and with a high performance liquid Chromatographic technique with spectrofluorimetric detection, exemplified on pine tissue (Pinus sylvestris L.). -Physiol. Plant.55: 315–322, 1982.
Zenk, M. N., Scherf, H. D.: D-tryptophan in höheren Pflanzen. -Biochim. biophys. Acta71: 737–739, 1963.
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Rekoslavskaya, N.I. Possible role of N-malonyl-D-tryptophan as an auxin precursor. Biol Plant 28, 62–67 (1986). https://doi.org/10.1007/BF02885326
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DOI: https://doi.org/10.1007/BF02885326