Summary
The effect of single and of multiple doses of prednisolone upon cell population kinetics in the rat jejunal crypt was investigated, using autoradiography and stathmokinetic techniques with vincristine. Single injections of prednisolone (2.5 mg/kg body weight) induced a depression in both flash thymidine labelling and mitotic indices; this change was shown to be due to a decreased cell production rate. Recovery of these proliferative indices occurred over seven days after injection; measurement of crypt size parameters showed a transient decrease in crypt population.
Multiple daily injections of prednisolone (1 mg/kg body weight) produced a more sustained decrease in labelling and mitotic indices, which lasted as long as injections were continued (7 days); stathmokinetic techniques showed decreases in cell production rates, and the crypt population was also depressed throughout this period.
It is concluded that prednisolone depresses cell proliferative rates in rat jejunal mucosa.
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Al-Dewachi, H.S., Wright, N.A., Appleton, D.R., Watson, A.J.: The cell cycle time in the rat jejunum. Cell Tiss. Kinet.7, 587–594 (1974)
Al-Dewachi, H.S., Wright, N.A., Appleton, D.R., Watson, A.J.: Cell population kinetics in the mouse jejunal crypt. Virchows Arch. B, Cell Path.18, 225–242 (1975)
Al-Dewachi, H.S., Wright, N.A., Appleton, D.R., Watson, A.J.: Studies on the mechanism of diurnal variation in the small bowel mucosa of the rat. Cell Tiss. Kinet.9, 459 467 (1976)
Batt, R.M., Peters, T.J.: Effects of prednisolone on the small intestinal mucosa of the rat. Clin. Sci. Mol. Med.50, 511–523 (1976)
Bullough, W.S.: Mitotic and functional homeostasis: a speculative review. Cancer Res.25, 1683–1727 (1965)
Cairnie, A.B., Bentley, R.E.: Cell proliferation studies in the intestinal epithelium of the rat; hyperplasia during lactation. Exp. Cell Res.46, 428–440 (1967)
Cairnie, A.B., Lamerton, L.F., Steel, G.G.: Cell proliferation studies in the intestinal epithelium of the rat. II Theoretical aspects. Exp. Cell Res.39, 539–553 (1965)
Dowling, R.H., Gleeson, M.H.: Cell turnover following small bowel resection and by-pass. Digestion8, 176–190 (1973)
Frankfurt, O.S.: Effect of hydrocortisone, adrenalin and actinomycin D on transition of cells to the DNA synthesis phase. Exp. Cell Res.52, 222–232 (1968)
Tannock, I.F.: A comparison of the relative efficiencies of the various metaphase arrest agents. Exp. Cell Res.47, 345–356 (1967)
Tutton, P.J.M.: Proliferation of epithelial cells in the jejunal crypts of adrenalectomised and adreno-cortical hormone treated rats. Virchows Arch. B Cell Path.23, 327–332 (1973)
Wall, A.J., Peters, J.J.: Changes in structure and peptidase activity of rat small intestine induced by prednisolone. Gut12, 445–448 (1971)
Wright, N.A., Al-Dewachi, H.S., Appleton, D.R., Watson, A.J.: Cell population kinetics in the rat jejunal mucosa. Cell Tiss. Kinet.8, 361–368 (1975)
Wright, N.A., Appleton, D.R., Morley, A.R.: The action of dexamethasone on cell population kinetics in the prepubertal rat adrenal cortex. J. Endocr.62, 527 536 (1974)
Wright, N.A., Morley, A.R., Appleton, D.R.: Variation in the duration of mitosis in the crypts of Lieberkühn of the rat; a cytokinetic study using vincristine. Cell Tiss. Kinet.5, 351–364 (1972a)
Wright, N.A., Morley, A.R., Appleton, D.R.: The effect of testosterone on cell proliferation and differentiation in the small bowel. J. Endocr.52, 161–175 (1972b)
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Wright, N.A., Al-Dewachi, H.S., Appleton, D.R. et al. The effect of single and of multiple doses of prednisolone tertiary butyl acetate on cell population kinetics in the small bowel mucosa of the rat. Virchows Arch. B Cell Path. 28, 339–350 (1978). https://doi.org/10.1007/BF02889082
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DOI: https://doi.org/10.1007/BF02889082