Summary
The urinary bladder epithelium contains nuclei with diploid, tetraploid and octoploid DNA content. A study in mice has been performed on mitotic activity, DNA synthesis and ploidy. The study has confirmed previous findings of an extremely low mitotic activity and a LI of 0.40±0.04%. The results indicate a higher DNA synthesis during the night as compared to the day.
The relative numbers of diploid-, tetraploid-and octoploid cells have been estimated by micro-flow fluorometry. The mean values of these different classes were diploid: 39.40±1.14%, tetraploid: 53.27 ±1.00% and octoploid: 4.41 ±0.22%. Hexaploid DNA values with a mean of 2.51 ±0.13% were found and the significance of hexaploid pulses is discussed.
The different ploidy classes changed during the neonatal period with a decrease of the diploid class and an increase of the tetraploid class. A reduction of the octoploid class about 4 weeks after birth was seen. The distribution of the different classes became stabilized around the second month.
Similar content being viewed by others
References
Alfert, M., Geschwind, I.I.: The development of polysomaty in rat liver. Exp. Cell Res.15, 230–232 (1958)
Berkhan, E.: DNS-Messung von Zellen aus Vaginalabstrichen. Ärztl. Lab.18, 77–79 (1972)
Clayson, D. B., Cooper, E. H.: The immediate response of bladder epithelium to injury by chemical carcinogens. Brit. J. Urol.41, 710–713 (1969)
Clayson, D. B., Cooper, E. H.: Cancer of the urinary tract. Advanc. Cancer Res.13, 271–381 (1970)
Clayson, D. B., Pringle, J. A. S.: The influence of a foreign body on the induction of tumours in the bladder epithelium of the mouse. Brit. J. Cancer20, 564–568 (1966)
Cooper, E. H.: The biology of bladder cancer. Ann. roy. Coll. Surg. Engl.51, 1–16 (1972)
Cooper, E. H., Levi, P. E., Anderson, C. K., Williams, R. E.: The evolution of tumour cell populations in human bladder cancer. Brit. J. Urol.41, 714–717 (1969)
Dittrich, W., Göhde, W.: Impulsfluorometrie bei Einzelzellen in Suspension. Z. Naturforsch.24b, 350–361 (1969)
Farsund, T.: Preparation of bladder mucosa cells for micro-flow fluorometry. Virchows Arch. Abt.B 16, 35–42 (1974)
Himes, M., Hoffman, J., Pollister, A. W., Post, J.: Origin of polyploid nuclei in rat livers during regeneration following carbon tetrachloride poisoning. J. Mt Sinai Hosp.24, 935–938 (1957)
Laerum, O. D., Maurer, H. R.: Proliferation kinetics of myelopoietic cells and macrophages in diffusion chambers after treatment with granulocyte extracts (chalone). Virchows Arch. Abt.B 14, 293–305 (1973)
Levi, P. E., Cowen, D. M., Cooper, E. H.: Induction of cell proliferation in the mouse bladder by 4-ethylsulphonyl-naphthalene-l-sulphonamide. Cell Tiss. Kinet.2, 249–262 (1969)
Locher, G. W., Cooper, E. H.: Repair of rat urinary bladder epithelium following injury by cyclophosphamide. Invest. Urol.8, 116–123 (1970)
Martin, B. F.: Cell replacement and differentiation in transitional epithelium: a histological and autoradiographic study of the guinea-pig bladder and ureter. J. Anat. (Lond.)112, 433–455 (1972)
Messier, B., Leblond, C. P.: Cell proliferation and migration as revealed by radioautography after injection of Thymidine-H3 into male rats and mice. Amer. J. Anat.106, 247–285 (1960)
Tvermyr, E. M. F.: Circadian rhythms in hairless mouse epidermal DNA-synthesis as measured by double labelling with H3-Thymidine (H3Tdr). Virchows Arch. Abt.B 11, 43–54 (1972)
Walker, B. E.: Polyploidy and differentiation in the transitional epithelium of mouse urinary bladder. Chromosoma (Berl.)9, 105–118 (1958)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Farsund, T. Cell kinetics of mouse urinary bladder epithelium. Virchows Arch. B Cell Path. 18, 35–49 (1975). https://doi.org/10.1007/BF02889232
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02889232