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Effect of ocimum flavonoids as a radioprotector on the erythrocyte antioxidants in oral cancer

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Abstract

Flavonoids extracted from the leaves of Indian holy basil, Ocimum sanctum showed promising results as radioprotector in rodents. Hence it was thought pertinent to analyze the antioxidants of erythrocytes in oral cancer patients who were concurrently treated with radiation and ocimum flavonoids. Oral cancer patients consisted of 2 groups. Group A (n=17) received radiation alone while Group B(n=17) received radiation and ocimum flavonoids(OF). Samples of heparinised blood were collected prior to treatment, 15 and 30 days respectively after treatment. Blood from normal healthy volunteers were taken as controls (n=25). Erythrocytes were analyzed for the antioxidants viz. glutathione, glucose 6 phosphate dehydrogenase, glutathione peroxidase and superoxide dismutase by the standard methods. Results of analysis indicated that erythrocytes from cancer patients (Group A and B) had significantly high glutathione levels before as well as after treatment compared to controls. It was observed that Group B which received OF showed a significant reduction in glutathione levels in comparison with Group A. All the other parameters showed no statistical significant changes. Results of the study suggest that erythrocytes from cancer patients responded to oxidative stress by elevating glutathione levels, while a decrease in glutathione levels observed in Group B, could be due to the free radical scavenging effect of OF, sparing the glutathione. However OF did not seem to exert its effect on other antioxidants of erythrocytes.

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References

  1. Frenkel K. (1992). Carcinogen mediated oxidant formation and oxidative DNA damage.Pharmacol. Ther. 53(1), 127–66.

    Article  PubMed  CAS  Google Scholar 

  2. Lasso de La vega MC, Terradenz P, Obrador E, et al. (1994). Inhibition of cancer growth and selective glutathione depletion in Ehrlich tumor cell in vivo by extracellular ATP.Biochem.J. 298 (Pt1), 99–105.

    PubMed  CAS  Google Scholar 

  3. Jaruga P, Olinski R. (1994). (Activity of antioxidant enzymes in cancer diseases) Postephy.Hig. Med. Dosw. 48(4), 1443–1455.

    Google Scholar 

  4. Sabitha KE, Shyamaladevi CS. (1999). Oxidant and antioxidant activity changes in patients with oral cancer and treated with radiotheraphy.Oral. Oncol. 35(3), 273-

    Google Scholar 

  5. Uma Devi P, Nagarathnam A, Satish Rao BS. In: Introduction to Radiation Biology 2000, 136.

  6. Devi P U, Ganasoundari A. (1999) Modulation of glutathione and antioxidant enzymes by ocimum sanctum and its role in protection against radiation injury.Ind. J. Exp. Biol. 37, 262–8.

    CAS  Google Scholar 

  7. Ganasoundari A, Uma Devi P and Rao MNA. (1997). Protection against radiation-induced chromosome damage in mouse bone marrow by ocimum sanctum.Mutat. Res. 373, 271–6.

    PubMed  CAS  Google Scholar 

  8. Karnofsky DA, Abelman Wh, Gaver IF, etal. (1948)Cancer 1834–656.9)

  9. Open MM, Greech RH, Tormy DC et al. (1982)Am. J. Clin. Oncol. 5, 649–655.

    Article  Google Scholar 

  10. Uma Devi P, Ganasoundari A, Rao BSS Srinivasan KK (1999). Invivo radioprotection by ocimum flavonoids; Survival of mice.Radiat. Res. 151, 74–78.

    Article  PubMed  CAS  Google Scholar 

  11. Kartha VN, Krishnamurthy S (1978). Factors affecting invitro lipid peroxidation of rat brain homogenate.Ind. J. Physiol. Pharmacol. 2244–52.

  12. Beutler E, Duron O, Kelley BM. (1963)J. Lab. Clin. Med. 61, 882.

    PubMed  CAS  Google Scholar 

  13. Paglia DE, Valentine WN. (1967). Studies on the qualitative and quantitative characterization of glutathione peroxidase.J. Lab. clin. Med. 70, 158.

    PubMed  CAS  Google Scholar 

  14. Lawrence RA, Burk RF. (1976) Glutathione peroxidase activity in selenium deficient rat liver.Biochem. Biophys. Res. Commun. 71, 952–958.

    Article  PubMed  CAS  Google Scholar 

  15. Sidney P. Colowick, Nathan D. Kaplan (1984) Methods in Enzymology vol 105. Acadamic Press, INC (London) Ltd, 364.

    Google Scholar 

  16. Beauchamp C. Fridovich I. (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.Anal. Biochem. 44, 276.

    Article  PubMed  CAS  Google Scholar 

  17. Mc.Cord JM, Fridovich I. (1969). Superoxide dismutase: an enzymatic function of erythrocuprien (hemocuprien).J. Biol. chem. 244, 6049.

    CAS  Google Scholar 

  18. Vorley H, Gowenlock AH, Bell M. (1980). Practical Clinical Biochemistry, 5th edition, Volume 1, William Heinemann Medical Books Ltd, London, 979.

    Google Scholar 

  19. Wong DY, Tsiao YL, Poon CK, Kivan PC et al. (1994). Glutathione concentration in oral cancer tissues.Cancer lett 30.81(2), 111–116.

    Article  Google Scholar 

  20. Krishnamurthy S. Jaya S. (1986). Serum alpha Tocopherol Lipo-peroxides and ceruloplasmin and red cell glutathione and antioxidant enzymes in patients of oral cancer.Ind. J. of cancer. 23, 36–42.

    CAS  Google Scholar 

  21. Bhattathiri VN, Sreelekha TT, Sebestian P, et al. (1994). Influence of plasma GSH level on acute radiation mucositis of the oral cavity.Int. J. Radiat. Oncol. Biol. Phys. 15; 29(2), 383–6.

    PubMed  CAS  Google Scholar 

  22. Stankiewiez A, Skrzydlewska E, Makiela M. (2002). Effects of amifostine on liver oxidative stress caused by cyclophosphamide administration to rats.Drug. Metabol. Drug. Interact. 19(2): 67–82.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Centre for Basic Sciences, Kasturba Medical College, 575 004, Mangalore

    Reshma K

  2. Department of Biochemistry, Father Muller’s Medical College, Mangalore

    Ashalatha V Rao

  3. Department of Oncology, Amrita Institute of Medical Sciences, Elamakkara, Cochin

    Dinesh M

  4. Amritha Institute Of Medical Sciences, Elamakkara, Cochin

    Vasudevan DM

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  1. Reshma K
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  2. Ashalatha V Rao
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Reshma, K., Ashalatha, V.R., Dinesh, M. et al. Effect of ocimum flavonoids as a radioprotector on the erythrocyte antioxidants in oral cancer. Indian J Clin Biochem 20, 160–164 (2005). https://doi.org/10.1007/BF02893064

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