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Removal of browning and growth enhancement by polyvinylpolypyrrolidone in protoplast cultures ofCyamopsis tetragonoloba L.

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Biologia Plantarum

Abstract

The occurrence of browning in protoplast cultures ofCyamopsis tetragonoloba completely inhibited the growth of protoplast derived colonies. Of the various additives employed to counteract the problem of browning and subsequent necrosis, polyvinylpolypyrrolidone (PVPP) was found most effective. Simultaneous addition of polyvinylpyrrolidone (PVP) to the protoplast culture medium accentuated the effect of PVPP and also improved the frequency of protoplast division.

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Author notes

  1. Praveen K. Saxena

    Present address: Department of Biology, University of Saskatchewan, S7N OWO, Saskatoon, Canada

Authors and Affiliations

  1. Department of Botany, University of Delhi, 1007, Delhi

    Praveen K. Saxena

  2. Bio-Organic Division, Bhabha Atomic Research Centre, Bombay, India

    R. Gill

Authors
  1. Praveen K. Saxena
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  2. R. Gill
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Saxena, P.K., Gill, R. Removal of browning and growth enhancement by polyvinylpolypyrrolidone in protoplast cultures ofCyamopsis tetragonoloba L.. Biol Plant 28, 313–315 (1986). https://doi.org/10.1007/BF02902302

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  • DOI: https://doi.org/10.1007/BF02902302

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