Abstract
Treatment of a flocculent (strain 201) and a non-flocculent (strain 116) cell suspension of Saccharomyces carlsbergensis with trypsin, followed by β-mercaptoethanol and a second trypsin treatment solubilized approximately 15% of material from both types of cells based on dry weight. The solubilized fractions were resolved by Sephadex gel filtration into high mol. wt. (40,000 to 200,000) and low mol.wt. (less than 4,000) components. High mol.wt. components from the flocculent cells contained more carbohydrate and less protein than the corresponding components isolated from the non-flocculent cells. Furthermore, the polypeptide material from the flocculent cells had a higher content of proline, lysine, and arginine than that from non-flocculent cells. High mol.wt. material contained antigenic determinants similar to those previously found in beer fractions. Yeast-derived antigens were found in all fractions while antigens of barley origin were found only in the tryptic digests.
A differential radioactive labelling technique demonstrated minor differences between the components released from flocculent and non-flocculent cell residues upon digestion with pronase.
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Marfey, P., Sørensen, S.B. & Ottesen, M. Studies on yeast flocculation. Comparison of enzymatic digests of flocculent and non-flocculent cells of Saccharomyces carlsbergensis. Carlsberg Res. Commun. 42, 353–367 (1977). https://doi.org/10.1007/BF02906120
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DOI: https://doi.org/10.1007/BF02906120