Abstract
The elemental composition of 10 methanogenic species was determined by inductively coupled plasma emission spectrometry and by a C-H-N-analyzer. The 10 species were representative of all three orders of the methanogens and were cultivated under defined conditions. Special emphasis was given toMethanosarcina barkeri, represented by 5 strains and cultivated on various substrates. The following elements with the lowest and highest values in parentheses were determined: C (37–44%, w/w), H (5.5–6.5%), N (9.5–12,8%); Na (0.3–4.0%), K (0.13–5.0%), S (0.56–1.2%), P (0.5–2.8%), Ca (order I: 85–550 ppm; order II: 1000–4500 ppm), Mg (0.09–0.53%), Fe (0.07–0.28%), Ni (65–180 ppm), Co (10–120 ppm). Mo (10–70 ppm), Zn (50–630 ppm), Cu (<10–160 ppm), Mn (<5–25 ppm). The biggest variations were found with respect to N and K, which both seem to have important physiological functions. Although it is unknown whether zinc and copper are essential trace elements for methanogens, all investigated species contained remarkably high zinc contents, whereas copper seemed to be present only in some species.
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References
W. E. Balch, G. E. Fox, L. J. Magrum, C. R. Woese, and R. S. Wolfe,Microbiol. Rev. 43, 260 (1979).
H. J. M. Bowen,Trace Elements in Biochemistry, Academic Press, New York, 1966.
G. Diekert, U. Konheiser, K. Piechulla, and R. K. Thauer,J. Bacteriol. 148, 459 (1981).
A. A. Esener, J. A. Roels, and N. W. F. Kossen,Biotechnol. Bioeng. 24, 1445 (1982).
E. M. Godsy,Appl. Environ. Microbiol. 39, 1074 (1980).
E.-G. Graf and R. K. Thauer,FEBS Lett. 136, 165 (1981).
D. Herbert, inMicrobial Reaction to Environment (11th Symp. Soc. General Microbiol.), Cambridge University Press, Cambridge, UK, 1961, pp. 391–416.
H. Hippe, D. Caspari, K. Fiebig, and G. Gottschalk,Proc. Natl. Acad. Sci. USA 76, 494 (1979).
T. J. Hutten, H. C. M. Bongaerts, C. van der Drift, and G. D. Vogels,Antonie van Leeuwenhoek 46, 601 (1980).
K. F. Jarrell and G. D. Sprott,Can. J. Microbiol. 27, 720 (1981).
K. F. Jarrell, J. R. Colvin, and G. D. Sprott,J. Bacteriol. 149, 346 (1982).
J. B. Jones and T. C. Stadtman,J. Bacteriol. 130, 1404 (1977).
G. E. Jones, L. G. Royle, and L. Murray,Appl. Environ. Microbiol. 38, 800 (1979).
O. Kandler and H. König,Arch. Microbiol. 118, 141 (1978).
T. W. Kirby, J. R. Lancaster, Jr., and I. Fridovich,Arch. Biochem. Biophys. 210 140 (1981).
F.-C. Kung, J. Raymond, and D. A. Glaser,J. Bacteriol. 126, 1089 (1976).
J. K. Lanyi,Biochem. Biophys. Acta 559, 377 (1979).
R. A. Mah, M. R. Smith, and L. Baresi,Appl. Environ. Microbiol. 35, 1174 (1978).
R. A. Mah and M. R. Smith, inThe Prokaryotes (M. P. Starr, H. Stolp, H. G. Trüper, A. Balows, and H. G. Schlegel, eds.), Springer-Verlag, Berlin, 1981, pp. 948–977.
G. B. Patel, and L. A. Roth,Can. J. Microbiol. 23, 893 (1977).
G. B. Patel, A. W. Khan, and L. A. Roth,J. Appl. Microbiol. 45, 347 (1978).
H.-J. Perski, J. Moll, and R. K. Thauer,Arch. Microbiol. 130, 319 (1981).
H.-J. Perski, P. Schönheit, and R. K. Thauer,FEBS Lett. 143, 323 (1982).
N. Pfennig and K. D. Lippert,Arch. Mikrobiol. 55, 245 (1966).
P. H. Rönnow and L. A. H. Gunnarsson,FEMS Microbiol. Lett. 14, 311 (1982).
P. A. Scherer and R. K. Thauer,Eur. J. Biochem. 85, 125 (1978).
P. Scherer and H. Sahm, inProceedings of the 1st International Symposium on Anaerobic Digestion, Cardiff, 1979, Poster Papers (D. A. Stafford and B. I. Wheatley, eds.), A. D. Scientific Press, Cardiff, 1980, pp. 45–47.
P. Scherer and H. Sahm,Eur. J. Appl. Microbiol. Biotechnol. 12, 28 (1981).
P. Scherer and H. Sahm,Acta Biotechnologica 1, 57 (1981).
P. Scherer, M. Kluge, J. Klein, and H. Sahm,Biotechnol. Bioeng. 23, 1057 (1981).
C. G. T. P. Schnellen, Thesis, Technical University of Delft, The Netherlands, (Rotterdam: De Maastad, publisher), 1947.
P. Schönheit, J. Moll, and R. K. Thauer,Arch. Microbiol. 123, 105 (1979).
G. D. Sprott and K. F. Jarrell,Can. J. Microbiol. 27, 444 (1981).
T. C. Stadtman, and B. A. Blaylock,Fed. Proc. 25, 1657 (1966).
C. H. Suelter, inCRC Handbook of Microbiology, vol. 4, 2nd ed., A. I. Laskin and H. A. Lechevalier, eds., CRC Press, Boca Raton, Florida, 1982, pp. 553–564.
F. P. Takacs, T. I. Matula, and R. A. MacLeod,J. Bacteriol. 87, 510 (1964).
G. D. Vogels, J. T. Keltjens, T. J. Hutten, and C. van der Drift,Zbl. Bakt. Hyg. I. Abt. Orig. C 3, 258 (1982).
W. B. Whitman, E. Ankwanda, and R. S. Wolfe,J. Bacteriol. 149, 852 (1982).
R. J. P. Williams,Q. Rev. 24, 331 (1970).
C. R. Woese,Sci. Amer. 244(6), 94 (1981).
E. A. Wolin, M. J. Wolin, and R. S. Wolfe,J. Biol. Chem. 238, 2882 (1963).
S. Yamazaki and L. Tsai,Fed. Proc. 39, p. 1698 (Abstr. 490) (1980).
S. Yamazaki and L. Tsai,J. Biol. Chem. 255, 6462 (1980).
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Scherer, P., Lippert, H. & Wolff, G. Composition of the major elements and trace elements of 10 methanogenic bacteria determined by inductively coupled plasma emission spectrometry. Biol Trace Elem Res 5, 149–163 (1983). https://doi.org/10.1007/BF02916619
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DOI: https://doi.org/10.1007/BF02916619
Index Entries
- Methanogenic bacteria, major and trace elements in
- Methanosarcina, determination of trace and major elements in
- sodium, in methanogenic bacteria
- potassium, in methanogenic bacteria
- magnesium, in methanogenic bacteria
- iron, in methanogenic bacteria
- cobalt, in methanogenic bacteria
- zinc, in methanogenic bacteria
- manganese, in methanogenic bacteria
- sulfur, in methanogenic bacteria
- phosphorus, in methanogenic bacteria
- nickel, in methanogenic bacteria
- molybdenum, in methanogenic bacteria
- copper, in methanogenic bacteria
- trace elements, determination in methanogenic bacteria
- elements, determination in methanogenic bacteria
- plasma emission spectroscopy, of elements in methanogenic bacteria
- emission spectroscopy, of elements in methanogenic bacteria
- spectroscopy, of elements in methanogenic bacteria
- bacteria, determination of elements in
- ICP, of methanogenic bacteria