Abstract
A simple model for membrane fusion mediated by vial spike glycoproteins is presented. The viral proteins are considered to be allosteric proteins that undergo concerted conformational transitions when they bind the ligand. The ligand in this case is H+. The effect of the conformational transition is to bring membranes together and induce their fusion. An equation is derived for the dependence of fusion rates on ligand concentration, for a given dissociation constant (K d), equilibrium constant for the conformational change (L), and number of cooperating subunits (n). Curves generated by this equation provide a reasonable fit to data on the rates of fusion of Vesicular Stomatitis virus with cells for a pK d of 6.3,L=1000 andn=6.
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Hendler, R. W. (1971),Physiol. Rev. 51, 66.
Monod, J., Wyman, J., and Changeux, J. P. (1965),J. Mol. Biol. 12, 88.
Changeux, J. P., Thiery, J., Tung, Y., and Kittel, C. (1976),Proc. Natl. Acad. Sci. USA 57, 335.
Hill, T. L. (1967),Proc. Natl. Acad. Sci. USA 58, 111.
Blumenthal, R., Changeux, J-P., and Lefever, R. (1970),J. Membr. Biol. 2, 351.
Cole, K. S. (1968),Membranes, Ions, and Impulses, University of California, Berkeley, pp. 1–569.
Hill, T. L. (1972), Comments on the Theory of Ion Transport Across the Nerve Membrane, inPerspectives in membrane biophysics; a tribute to Kenneth S. Cole (Agin, D. P., ed.), Gordon and Breach, New York, pp. 187–203.
Hill, T. L., and Chen, Y. (1970),Proc. Natl. Acad. Sci. USA 65, 1069.
Blumenthal, R. (1987),Current Topics on Membranes and Transport,29, 203.
White, J., Kielian, M., and Helenius, A. (1983),Q. Rev. Biophys. 16, 151.
Skehel, J. J., Bayley, P. M., Brown, E., Martin, S. R., Waterfield, M. D., White, J. M., Wilson, I. A., and Wiley, D. C. (1982),Proc. Natl. Acad. Sci. USA,79, 968.
Dales, S. (1973),Bacteriol. Rev. 37, 103.
Matlin, K. S., Reggio, H., Helenius, A., and Simons, K. (1982),J. Mol. Biol. 156, 609.
White, J., Matlin, K., and Helenius, A. (1981),J. Cell Biol. 89, 674.
Hoekstra, D., de Boer, T., Klappe, K., and Wilschut, J. (1984),Biochemistry 23, 5675.
Blumenthal, R., Bali-Puri, A., Walter, A., Covell, D., and Eidelman, O. (1987),J. Biol. Chem.,262, 13614.
Florkiewicz, R. Z., and Rose, J. K. (1984),Science 225, 721.
Thomas, D., Newcomb, W. W., Brown, J. C., Wall, J. S., Hainfeld, J. F., Trus, B. L., and Steven, A. C. (1985),J. Virol. 54, 598.
Crimmins, D. L., Mehard, W. B., and Schlesinger, S. (1983),Biochemistry 22, 5790.
Ehrenstein, G., Glumenthal, R., Latorre, R., and Lecar, H. (1974),J. Gen. Physiol. 63, 707.
Poste, G., and Pasternak, C. A. (1978),Cell Surf. Rev. 5, 305.
Gething, M. J., Doms, R. W., York, D., and White, J. (1986),J. Cell Biol.,102, 11.
Eidelman, O., Schlegel, R., Tralka, T. S., and Blumenthal, R. (1984),J. Biol. Chem. 259, 4622–4628.
Barrowman, M. M., Cockcroft, S., and Gomperts, B. D. (1986),Nature 319, 504.
Szabo, Z. (1969), Kinetic Characterization of Complex Reaction Kinetics, inComprehensive Chemical Kinetics (Bamford, C. H., and Tipper, C. F. H., eds.), Elsevier, Amsterdam, vol. 2, pp. 24–26.
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Blumenthal, R. Cooperativity in viral fusion. Cell Biophysics 12, 1–12 (1988). https://doi.org/10.1007/BF02918347
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DOI: https://doi.org/10.1007/BF02918347