Abstract
Metabolites of indolepyruvic acid and indolelactic acid were investigated using 2 systems: a bacterial (pea stem homogenates containing the epiphytic bacteria) and a plant system (pea stem sections under sterile conditions). The products of spontaneous indolepyruvic acid decomposition in aqueous solution and during chromatography were investigated, too.
Biological indolepyruvic acid conversion yields, besides those substance amounts which occur spontaneously, indoleacetic acid, indoleethanol (tryptophol) and (only in the sterile plant system) indoleacetaldehyde. An inhibitor extract from pea stems decreases the indoleacetic acid and increases the indoleethanol and indoleacetaldehyde gain.
Indolelactic acid is not metabolized in the sterile plant sections. Indolelactic acid oxidation by the bacteria-containing homogenate yields indolepyruvic acid and is inhibited by the inhibitor extract.
Abstract
Metabolity kys. indolylpyrohroznové a kys. indolylmléčné byly studovany za pomoci 2 systémů: bakteriálního (homogenáty stonků hrachu obsahujících epifytické bakterie) a rostlinného (sekce stonků hrachu vypestovaného za sterilních podmínek). V laboratorních podmínkach byly rovnež sledovány produkty spontanního rozkladu kyseliny indolylpyrohroznové ve vedném roztoku a v průběhu chromatografie.
Biologická přeměna kys. indolylpyrohroznové poskytuje vedle látek, které se tvoří spontánně, kyselinu indolyloctovou, indolylethanol (tryptofol) a (jen ve sterilním prostředí) indolylacetaldehyd. Extrakt inhibitoru ze stonků hrachu snižuje kys. indolyloctovou a zvyšuje tvorbu indolylethanolu a indolylacetaldehydu. Kys. indolylmléčná není metabolizována ve sterilních sekcích rostlin. Oxydací kys. indolylmléčné homogenátem obsahujícím bakterie se tvoří kys. indolylpyrohroznová; tato oxydace je inhibována extraktem s nativním inhibitorem.
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Libbert, E., Brunn, K., Drawert, A. et al. Pathways of IAA production from tryptophan by plants and by their epiphytic bacteria: Metabolism of indolepyruvic acid and indolelactic acid. Biol Plant 12, 246–255 (1970). https://doi.org/10.1007/BF02920809
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DOI: https://doi.org/10.1007/BF02920809