Abstract
The roots ofAllium cepa L. were treated with water solutions of cyanein and griseofulvin for 24 and 48 h respectively, thereafter cultured for 48 h in the medium without the antibiotics, and the reversibility of the inhibition caused by the antibiotics was evaluated. Changes in the mitotic index of meristematic cells were followed in squash preparations of root apices. In addition to the cytologic observations, the differentiation of the primary meristem was followed in longitudinal sections. After the treatment with reversible doses of cyanein and griseofulvin respectively, differences were found in both the effects of the antibiotics during the treatment and the restoration of the inhibited processes after the treatment. In the cells treated with cyanein the restoration of mitotic activity was instantaneous but less intensive and in|complete especially in the case of the 48 h treatment. After the treatment with griseofulvin which temporarily interfered also with the differentiation of the primary meristem, the restoration of the inhibited processes was delayed but intensive after both application times, 48 h after the treatment no symptoms of the preceding inhibition could be observed.
Abstract
Na korienkyAllium cepa L. sa pôsobilo vodnými roztokmi cyaneinu a grizeofulvínu. Po 24 a 48 h ovlivňovania boli korienky 48 h kultivované v prostredí bez antibiotík a hodnotila sa reverzibilnost vyvolanej inhibície. Pri použití roztlakových preparátov koreňových vrcholkov sa sledovali hlavne zmeny mitotického indexu meristematických buniek. Cytologické pozorovania boli doplnené sledovaním diferenciácie primárneho meristému na pozdlžnych rezoch. Okrem rozdielnych účinkov pozorovaných počas priameho pôsobenia antibiotík sa po reverzibilných dávkach cyaneinu a grizoefulvínu prejavili podstatné rozdiely aj v odlišnom priebehu obnovy inhibovaných procesov. U buniek ovlivnených cyaneinom bola obnova mitotickej aktivity okamžitá, avšak obzvlášt po 48 h aplikácii málo intenzívna a preto neúplná. Po grizeofulvíne, ktorý prechodne narušil aj diferenciáciu primárneho meristému, bola obnova inhibovaných procesov po oboch aplikačných dobách oneskorená ale intenzívna a 48 h po ovliňovaní neboli príznaky predchádzajúcej inhibície pozorované.
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Frank, V. Restoration of mitotic and differentiation processes in the root apices ofAllium cepa L. treated with cyanein and griseofulvin. Biol Plant 16, 28–34 (1974). https://doi.org/10.1007/BF02920817
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DOI: https://doi.org/10.1007/BF02920817