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Development of embryoids in carrot root callus culture (Daucus carota L.)

Indukee tvorby embryoidů v kalusovém pletivu z kořene mrkve (Daucus carota L.)

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Biologia Plantarum

Abstract

Embryogenesis occurred in carrot root callus (Daucus carota L.) cultivated on simple synthetic medium containing IAA and 2,4-D. Embryoid development continued also during successive years when the tissue was cultivated on the same nutrient medium without those substances. Sometimes production of plants with atypical leaves was also observed. In those plants development of adventive embryoids occurred repeatedly. Result of this work confirmed reports about the organogenic potentiality of this species and about its sensitivity to some chemical substances.

Abstract

Při kultivaci z kalusového pletiva z kořene mrkve (Daucus carota L.) na jednoduché synthetické půdě, do které byly přidány kys. β indolyloctová a 2,4-D, nastala v pletivu embryogenesa. Tvorba embryoidů pokračovala i v dalších létech, když pletivo bylo pěstováno na stejné živné půdě bez těchto látek. Občas byla pozorována tvorba rostlinek satypickými listy. Na těchto rostlinách docházelo kopětné tvorbě adventivních, embryoidů. Výsledky práce potvrdily údaje o organogenní schopnosti tohoto druhu a jeho citlivosti vůči některým chemickým látkám.

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Authors and Affiliations

  1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha

    Eva Petrů

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  1. Eva Petrů
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Address: Ul. 1. listopadu 1050/9, Praha 4-Nusle, Czechoslovakia.

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Petrů, E. Development of embryoids in carrot root callus culture (Daucus carota L.). Biol Plant 12, 1–5 (1970). https://doi.org/10.1007/BF02920872

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  • DOI: https://doi.org/10.1007/BF02920872

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