Comparative electrophoretic investigation of some enzymes in extracts from different growth zones ofVicia faba L. Root

Abstract

Activities of five enzymes were followed in electrophoreograms of extracts from three growth zones of broad bean root. With ascorbate oxidase (L-ascorbate: oxygen oxidoreductase, E.C. 1.10.3.3) one new fraction was found in the electrophoreograms of extracts from the maturation zone, electrophoreograms of α-glucan phosphorylase (α-l,4-glucan: orthophosphate glucosyltransferase, E.C. 2.4.1.1), amylase (α-l,4-glucan 4-glucanohydrolase, E.C. 3.2.1.1), sucrose glucosyltransferase (disaccharide glucosyltransferase, E.C. 2.4.1.7) and leucine aminopeptidase (L-leucyl-peptide hydrolase, E.C. 3.4.1.1) were identical for all three investigated growth zones. Ascorbate oxidase activity occurred in the same regions of electrophoreograms as peroxidase activity.

Abstract

Na elektroforeogramech extraktů segmentů ze tři růstových Zon kořenu byly sledovány aktivity pěti enzymů. U oxydůzy kyseliny askorbove byla na elektroforeogramech extraktu ze zony s ukončeným dlouivym rustem zjistena jedna frakce navic, elektroforeogramy ostatnichctyf enzymu byly pro vsechny tfi sledovane zony kofenu identicke. Aktivita oxydazy kyseliny askorbove se vyskytovala ve stejnych oblasteoh elektroforeogramů jako aktivita peroxid7#x00E1;zy.