Abstract
Ethylene was collected in methanol solution of mercuric acetate and the addition compound formed was then separated by means of paper chromatography. The spot area and colour intensity after detection were determined using a densitometer. The amount of collected ethylene was calculated from a calibration curve.
The ethylene liberated from plant samples was collected during one or two days. During this period the amputation of the whole plant organs did not influence ethylene production. Changes in ethylene production were found after segmentation of the tissue or after the treatment with auxin and Co2+ ions.
The above-ground parts of investigated herbs released 0.3 to 3.5 μl of ethylene per kg fresh weight per hour. The leaves of investigated trees released 1 to 20 μl of ethylene per kg f.w. per hour. The rate of the production of ethylene seems to be specific for a given species.
Abstract
Pro kvantitativní analýzu produkce etylénu rostlinami bylo použito metody založené na jímání etylénu v metanolovém roztoku octanu rtuťnatého s následným dělením vzniklého komplexu pomocí papírové chromatografie. Velikost a intenzita zbarvení chromatografických skvrn byla po detekci stanovena pomocí densitometru. Množství etylénu bylo stanoveno odečtením z kalibrační křivky sestrojené ze standardních vzorků čistého etylénu.
Etylén uvolňovaný rostlinnými vzorky byl jímán v průběhu 1 až 2 dnů. Amputace celých orgánů rostlin neovlivnila během této doby produkci etylénu. Změny produkce etylénu byly zaznamenány po segmentaci tkáně či po infiltraci některých účinných látek.
Nadzemní části testovaných bylin uvolňovaly etylén přibližní v množství od 0,3 do 3,5 μl na kg čerstvé váhy za hod. Listy testovaných dřevin uvolňovaly etylén v množství cca od 1 do 20 μl na kg čerstvé váhy za hod. Intenzita produkce etylénu u rostlin se zdá být druhově specifická.
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Plhák, F., Souček, J. The production of ethylene by plants determined by means of paper chromatography. Biol Plant 15, 250–255 (1973). https://doi.org/10.1007/BF02922705
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DOI: https://doi.org/10.1007/BF02922705