Abstract
Drugs likeL-ethionine, 1,10-phenanthroline and 3-(2-thienyl)-DL-alanine which arrestSaccharomyces cerevisiae cells in the G1 phase, were unable to arrestCandida albicans cells. However,C. albicans could be arrested in G1 after a prolonged stationary phase. As compared to normal cells, there was a selective reduction in the level of accumulation of valine and glutamate in G1-arrested cells, while the phospholipid polar head group ratio was not significantly altered. When G1-arrestedC. albicans cells were again allowed to grow, the level of different phospholipids started increasing at about the time of bud emergence (2.5 h) whereas reduced levels of accumulated valine and glutamate recovered within 1 h. The recovery of phospholipids and amino acid transport are two distinct events during the progression ofG. albicans cells from G1 to S phase.
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References
Bedard D.P., Singer R.A., Johnston G.C.: Transient cell cycle arrestof Saccharomyces cerevisiae by amino acid analogue β-2-DL-thienylalanine.J. Bact. 141, 100 (1980).
Bedell G.W., Werth A., Soll D.R.: The regulation of nuclear migration and division during synchronous bud formation in released stationary phase cultures ot the yeastCandida albicans. Exp.Cell Res. 127, 103(1980).
Bradford M.M.: A rapid and sensitive method for the quantitation of micrograms quantities protein utilising the principle of protein dye binding.Anal. Biochem. 72, 248 (1976).
Brooks R.F.: The kinetics of serum induced initiation of DNA synthesis inBHK 21/C13 cells and the influence of exogenous adenosine.J.Cell.Physiol. 86, 369 (1975).
Cottrell S.F., Getz G.S., Rabinow’itz M.: Phospholipid accumulation during the cell cycle in synchronous cultures of the yeastSaccharomyces cerevisiae.J.Biol.Chem. 256, 10973 (1981).
Dudani A.K., Trivedi A., Prasad R.: The possible functional significance of phosphatidylinositol in G1 arrest ofSaccharomyces cerevisiae.FEBS Lett. 153, 34 (1983).
Gates B.J., Friedkein M.: Mid G1 marker protein(s) in 3T3 mouse fibroblast cells.Proc.Nat. Acad.Sci.USA 75, 4959 (1978).
Hartwell L.H.:Saccharomyces cerevisiae cell cycle.Bacteriol.Rev. 38, 164 (1974).
Haktwell L.H., Cullotti J., Pringle J.R., Reid B.J.: Genetic control of cell division cycle in yeast.Science 183, 46 (1974).
Jayakumar A., Singh M., Prasad R.: Characteristics of proline transport in normal and starved cells ofCandida albicans.Biochim.Biophys.Acta 514, 348 (1978).
Jayakumar A., Singh M., Prasad R.: An inducible proline transport system inCandida albicans.Biochim.Biophys.Acta 556, 144 (1979).
Johnston G.C., Singer R.A.: RNA synthesis and control of cell division in the yeastSaccharomyces cerevisiae. Cell 14, 951 (1978).
Kay D.C., Singer R.A., Johnston G.G.: Ornithine decarboxylase activity and cell cycle regulation inSaccharomyces cerevisiae.J. Bact. 141, 1041 (1980).
Pledger W.J., Stiles C.D., Antoniades H.N., Scher C.B.: An ordered sequence of events is required before Balb/3T3 cells become committed to DNA synthesis.Proc.Nat.Acad.Sci.USA 75, 2839 (1978).
Quinlan D.C., Hochstadt J.: The regulation by fibroblast growth factor of early transport changes in quiescent 3T3 cells.J.Cell. Physiol. 93, 237 (1977).
Riddle V.G.H., Dubrow R., Pardee A.B.: Changes in the synthesis of actin and other cell proteins after stimulation of serum arrested cells.Proc. Nat. Acad. Sci.USA 76, 1298 (1979).
Shilo B., Riddle V.G.H., Pardee A.B.: Protein turnover and cell cycle initiation in yeast.Exp.Cell Res. 123, 221 (1979).
Simohen G.: Cell division cycle mutants.Ann.Rev Genet. 12, 161 (1978).
Singer R.A., Johnston G.C., Bedabd D.: Methionine analogues and cell division regulation in the yeastSaccharomyces cerevisiae.Proc.Nat.Acad.Sci.USA 75, 6083 (1978).
Singh M., Jayakumab A., Prasad R.: The effect of altered lipid composition on the transport of various amino acids inCandida albicans.Arch. Biochem.Biophys. 191, 680 (1978).
Singh M., Jayakumar A., Prasad R.: The effect of altered ergosterol content on the transport of various amino acids inCandida albicans.Biochim. Biophys. Acta 555, 42 (1979).
Singh M.: Relationship between lipid composition and cellular permeability of yeast cells.PhD Thesis, Jawaharlal Nehru University, New Delhi (India) 1979.
Soll D.R., Bedell G., Thiel J., Brummel M.: The dependency of nuclear division on volume in the dimorphic yeast,Candida albicans. Exp.Cell Res. 133, 55 (1981).
Thomas K.C.: Effect of pH on the rate ofCandida utilis cell cycle initiation.J. Bad. 144, 1193 (1980).
Trivedi A., Khare S., Singhal G.S., Prasad R.: Effect of phosphatidylcholine and phosphatidylethanolamine enrichment on the structure and function of yeast membrane.Biochim. Biophys.Acta 692, 202 (1982).
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This work was supported in part by a grant from theIndian. Council of Medical Research (5/3-l(8)/81-BMS). The first author acknowledge Junior Research Fellowship award fromUniversity Grants Commission, India.
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Dttdani, A.K., Prasad, R. Differences in amino acid transport and phospholipid contents during the cell cycle ofCandida albicans . Folia Microbiol 30, 493–500 (1985). https://doi.org/10.1007/BF02927612
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DOI: https://doi.org/10.1007/BF02927612