Abstract
The genome ofAzotobacler vinelandii has been taggedin vivo with transposons. The cells have then been allowed to divide and the pattern of segregation of the genomes has been studied. The results suggest the presence of multiple (possibly identical) copies of the genome inA. vinelandii. Only a fraction of the total number of genomes seem to have been tagged with transposon and an equilibrium between alleles of the same gene with and without the transposon was evident during random segregation.
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References
Boyer H W and Roullard-Dussiox D 1969 A complementation analysis of the restriction and modification of DNA inEscherichia coli.J. Mol. Biol. 41: 459–472
Denhardt D 1976 A membrane filter technique for the detection of complementary DNA.Biochem. Biophys. Res. Commun. 23: 641–646
Kahn M, Kolter R, Thomas C, Figurski D, Meyer R, Remaut E and Helinski D R 1979 Plasmid cloning vehicles derived from plasmids Col El, F, R6K and RK2. InMethods in enzymology (ed.) R Wu (New York: Academic Press) vol. 68, pp. 268–280
Medhora M, Phadnis S H and Das H K 1983 Construction of a gene library from the nitrogen-fixing aerobeAzolobacter vinelandii.Gene 25: 355–360
Page W J and Sadoff H L 1976 Physiological factors affecting transformationof Azolobacter vinelandii.J. Bacteriol. 125: 1080–10877
Phadnis S H and Das H K 1987 Use of the plasmid pRK 2013 as a vehicle for transposition inAzolobacter vinelandii.J. Biosci. 12: 131–135
Ramos J L and Robson R L 1985 Isolation and properties of mutants ofAzolobacter chroococum defective in aerobic nitrogen fixation.J. Gen. Microbiol. 131: 1449–1458
Rigby P W J, Dieckmann M, Rhodes C and Berg P 1977 Labelling deoxyribonucleic acid to high specific activityin vitro by nick translation with DNA polymerase 1.J. Mol. Biol. 113: 237–251
Sadoff H L, Berke E and Loperfido B 1971 Physiological studies of encystment inAzolobacter vinelandii.J. Bacteriol. 105: 185–189
Sadoff H L, Shimel B and Ellis S 1979 Characterization ofAzolobacter vinelandii deoxyribonucleic acid and folded chromosomes,J. Bacteriol. 138: 871–877
Shah V K, Davis L C, Gordon J K, Orme-Johnson W H and Brill W J 1973 Nitrogenase III. Nitrogenaseless mutants ofAzotobacter vinelandii. Activities, cross reactions and EPR spectra.Biochim. Biophys. Acta 292: 246–255
Southern E M 1975 Detection of specific sequences among DNA fragments separated by gel electrophoresis.J. Mol. Biol. 98: 503–517
Thomas P S 1980 Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proc. Natl. Acad. Sci. USA 77: 5201–5205
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Phadnis, S.H., Dimri, G.P. & Das, H.K. Segregation characteristics of multiple chromosomes ofAzotobacter vinelandii . J. Genet. 67, 37–42 (1988). https://doi.org/10.1007/BF02927736
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DOI: https://doi.org/10.1007/BF02927736