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Screening ofMedicago wild species for callus formation and the genetics of somatic embryogenesis

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Abstract

Genotypes from twelve accessions of five wild species from the genusMedicago were screened for callus formation and somatic embryogenesis using two tissue culture protocols. These wild species were compared with two highly regenerable genotypes ofMedicago saliva. Embryogenesis for the wild types was very low. Wild species genotypes, which gave some callus production, were isolated.

In alfalfa previous studies have indicated that embryogenesis is genetically controlled. Of the two pairs of reciprocal crosses tested here, one provided evidence of cytoplasmic inheritance of the trait, the other did not. The parent which carried the cytoplasmic factor was A70.34. Nuclear factors are also involved in the inheritance of embryogenesis.

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Authors and Affiliations

  1. Plant Science Department, University of Alberta, T6G 2P5, Edmonton, Alberta, Canada

    P. D. Walton

  2. Genetic Engineering Section, Plant Research Centre, Agriculture Canada, 0C6, Ottawa, K1A, Canada

    D. C. W. Brown

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  1. P. D. Walton
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  2. D. C. W. Brown
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Walton, P.D., Brown, D.C.W. Screening ofMedicago wild species for callus formation and the genetics of somatic embryogenesis. J. Genet. 67, 95–100 (1988). https://doi.org/10.1007/BF02927789

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  • DOI: https://doi.org/10.1007/BF02927789

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