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Screening ofExiguobacterium acetylicum from soil samples showing enantioselective and alkalotolerant esterase activity

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Abstract

About 3,000 bacterial colonies with esterase activities were isolated from soil samples by enrichment culture and halo-size on Luria broth-tributyrin (LT) plates. The colonies were assayed for esterase activity in microtiter plates using enantiomerically pure (R)- and (S)-2-phenylbutyric acid resorufin ester (2PB-O-res) as substrates. Two enantioselective strains (JH2 and JH13) were selected by the ratio of initial rate of hydrolysis of enantiomerically pure (R)- and (S)-2-PB-O-res. When cell pellets were used, both strains showed hgh apparent enantioselectivity (E app>100) for (R)-2PB-O-res and were identified asExiguobacterium acetylicum. The JH13 strain showed high esterase activity onp-nitrophenyl acetate (pNPA), but showed low lipase activity onp-nitrophenyl palmitate (pNPP). The esterase was located in the soluble fraction of the cell extract. The crude intracellular enzyme preparation was stable at a pH range from 6.0 to 11.0.

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Authors and Affiliations

  1. Institute for Molecular Biology and Genetics, Seoul National University, 151-742, Seoul, Korea

    Bum-Yeol Hwang, Ji-Hyun Kim, Juhan Kim & Byung-Gee Kim

  2. School of Chemical Engineering, Seoul National University, 151-742, Seoul, Korea

    Bum-Yeol Hwang, Ji-Hyun Kim, Juhan Kim & Byung-Gee Kim

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  1. Bum-Yeol Hwang
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  2. Ji-Hyun Kim
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  4. Byung-Gee Kim
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Correspondence to Byung-Gee Kim.

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Hwang, BY., Kim, JH., Kim, J. et al. Screening ofExiguobacterium acetylicum from soil samples showing enantioselective and alkalotolerant esterase activity. Biotechnol. Bioprocess Eng. 10, 367–371 (2005). https://doi.org/10.1007/BF02931857

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  • DOI: https://doi.org/10.1007/BF02931857

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