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Characterization of a newly isolatedcis-1,2-dichloroethylene and aliphatic compound-degrading bacterium,Clostridium sp. strain KYT-1

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Abstract

Acis-1,2-dichloroethylene (cis-DCE)-degrading anaerobic bacterium,Clostridium sp. strain KYT-1, was isolated from a sediment sample collected from a landfill site in Nanji-do, Seoul, Korea. The KYT-1 strain is a gram-positive, endospore-forming, motile, rod-shaped anaerobic bacterium, of approximately 2.5∼3.0 μm in length. The degradation ofcis-DCE is closely related with the growth of the KYT-1 strain, and it was stopped when the growth of the KYT-1 strain became constant. Although the pathway ofcis-DCE degradation by strain KYT-1 remains to be further elucidated, no accumulation of the harmful intermediate, vinyl chloride (VC), was observed during anaerobiccis-DCE degradation. Strain KYT-1 proved able to degrade a variety of volatile organic compounds, including VC, isomers of DCE (1,1-dichloroethylene,trans-1,2-dichloroethylene, andcis-DCE), trichloroethylene, tetrachloroethylene, 1,2-dichloroethane, 1,1,1-trichloroethane, and 1,1,2-trichloroethane. Strain KYT-1 degradedcis-DCE at a range of temperatures from 15 to 37°C, with an optimum at 30°C, and at a pH range of 5.5 to 8.5, with an optimum at 7.0.

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Authors and Affiliations

  1. United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, 501-1193, Gifu, Japan

    Eun-Sook Kim & Kazuhiro Takamizawa

  2. Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, 501-1193, Gifu, Japan

    Izumi Nomura, Yuki Hasegawa & Kazuhiro Takamizawa

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  1. Eun-Sook Kim
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  2. Izumi Nomura
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  3. Yuki Hasegawa
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  4. Kazuhiro Takamizawa
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Correspondence to Kazuhiro Takamizawa.

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Kim, ES., Nomura, I., Hasegawa, Y. et al. Characterization of a newly isolatedcis-1,2-dichloroethylene and aliphatic compound-degrading bacterium,Clostridium sp. strain KYT-1. Biotechnol Bioproc E 11, 553–556 (2006). https://doi.org/10.1007/BF02932083

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  • DOI: https://doi.org/10.1007/BF02932083

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