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Removal of aqueous pentachlorophenol by horseradish peroxidase in the presence of surfactants

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Abstract

An important issue in the oxidation of pentachlorophenol (PCP) by the enzyme horseradish peroxidase (HRP) is enzyme inactivation during the reaction. This study was initiated to investigate the ability of two nonionic surfactants (Tween 20 and Tween 80) to mitigate HRP inactivation. The surfactants were tested at concentrations below and above their critical micelle concentrations (CMCs). Enhancement of PCP oxidation was observed at sub-CMCs, indicating effective protection of HRP by the two surfactants. Maximum levels of PCP removal were observed when the concentrations of Tween 20 and Tween 80 were 40 and 50% of the CMCs, respectively. At supra-CMCs, both surfactants caused a noticeable reduction in the extent of PCP removal.

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References

  1. Durán, N. and E. Esposito (2000) Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment: a review.Appl. Catal. B: Environ. 28: 83–99.

    Article  Google Scholar 

  2. Torres, E., I. Bustos-Jaimes, and S. Le Borgne (2005) Potential use of oxidative enzymes for the detoxification of organic pollutants.Appl. Catal. B: Environ. 46: 1–15.

    Article  Google Scholar 

  3. Tatsumi, K., S. Wada, and H. Ichikawa (1996) Removal of chlorophenols from wastewater by immobilized horseradish peroxidase.Biotechnol. Bioeng. 51: 126–130.

    Article  CAS  Google Scholar 

  4. Choi, Y. J., H. J. Chae, and E. Y. Kim (1999) Steady-state oxidation model by horseradish peroxidase for the estimation of the non-inactivation zone in the enzymatic removal of pentachlorophenol.J. Biosci. Bioeng. 88: 368–373.

    Article  CAS  Google Scholar 

  5. Zhang, G. and J. A. Nicell (2000) Treatment of aqueous pentachlorophenol by horseradish peroxidase and hydrogen peroxide.Water Res. 34: 1629–1637.

    Article  CAS  Google Scholar 

  6. Song, H. Y., J. Z. Liu, Y. H. Xiong, L. P. Weng, and L. N. Ji (2003) Treatment of aqueous chlorophenol by phthalic anhydride-modified horseradish peroxidase.J. Mol. Catal., B Enzym. 22: 37–44.

    Article  CAS  Google Scholar 

  7. Davidenko, T. I., O. V. Osevchuk, O. V. Sevastyanov, and I. I. Romanovskaya (2004) Peroxidase oxidation of phenols.Appl. Biochem. Microbiol. 40: 542–546.

    Article  CAS  Google Scholar 

  8. Ricotta, A., R. F. Unz, and J. M. Bollag (1996) Role of a laccase in the degradation of pentachlorophenol.Bull. Environ. Comam. Toxicol. 57: 560–567.

    Article  CAS  Google Scholar 

  9. Cho, N. S., J. Rogalski, M. Jaszek, J. Luterek, M. Wojtas-Wasilewska, E. Malarezyk, M. Fink-Boots, and A. Leonowicz (1999) Effect of coniferyl alcohol addition on removal of chlorophenols from water effluent by fungal laccase.J. Wood Sci. 45: 174–178.

    Article  CAS  Google Scholar 

  10. Ullah, M. A., C. T. Bedford, and C. S. Evans (2000) Reactions of pentachlorophenol with laccase fromCoriolus versicolor.Appl. Microbiol. Biotechnol. 53: 230–234.

    Article  CAS  Google Scholar 

  11. Wang, P., C. A. Woodward, and E. N. Kaufman (1999) Poly (ethylene glycol)-modified ligninase enhances pentachlorophenol biodegradation in water-solvent mixtures.Biotechnol. Bioeng. 64: 290–297.

    Article  CAS  Google Scholar 

  12. Choi, S. H., S. H. Moon, and M. B. Cu (2002) Biodegradation of chlorophenols using the cell-free culture broth ofPhanerochaete chrysosporium immobilized in polyurethane foam.J. Chem. Technol. Biotechnol. 77: 999–1004.

    Article  CAS  Google Scholar 

  13. Davila-Vazquez, G., R. Tinoco, M. A. Pickard, and R. Vazquez-Duhalt (2005) Transformation of halogenated pesticides by versatile peroxidase fromBjerkandera adusta.Enzyme Microb. Technol. 36: 223–231.

    Article  CAS  Google Scholar 

  14. Nakamoto, S. and S. Machida (1992) Phenol removal from aqueous solution by peroxidase-catalyzed reaction using additives.Water Res. 26: 49–54.

    Article  CAS  Google Scholar 

  15. Wu, J., K. E. Taylor, J. K. Bewtra, and N. Biswas (1993) Optimization of the reaction conditions for enzymatic removal of phenol from wastewater in the presence of polyethylene glycol.Water Res. 27: 1701–1706.

    Article  CAS  Google Scholar 

  16. Wu, Y., K. E. Taylor, N. Biswas, and J. K. Bewtra (1997) Comparison of additives in the removal of phenolic compounds by peroxidase-catalyzed polymerization.Water Res. 31: 2699–2704.

    Article  CAS  Google Scholar 

  17. Wu, Y., K. E. Taylor, N. Biswas, and J. K. Bewtra (1998) A model for the protective effect of additives on the activity of horseradish peroxidase in the removal of phenol.Enzyme Microb. Technol. 22: 315–322.

    Article  CAS  Google Scholar 

  18. Wu, Y., K. E. Taylor, N. Biswas, and J. K. Bewtra (1999) Kinetic model for removal of phenol by horseradish peroxidase with PEG.J. Environ. Eng. 125: 451–458.

    Article  CAS  Google Scholar 

  19. Villalobos, D. A. and I. D. Buchanan (2002) Kemoval of aqueous phenol byArthromyces ramosus peroxidase.J. Environ. Eng. Sci. 1: 65–73.

    Article  CAS  Google Scholar 

  20. Sakurai, A., M. Masuda, and M. Sakakibara (2003) Effect of surfactants on phenol removal by the method of polymerization and precipitation catalyzed byCoprinus cinereus peroxidase.J. Chem. Technol. Biotechnol. 78: 952–958.

    Article  CAS  Google Scholar 

  21. Patist, A., S. S. Bhagwat, K. W. Penfield, P. Aikens, and D. O. Shah (2000) On the measurement of critical micelle concentrations of pure and technical-grade nonionic surfactants.J. Surfact. Deterg. 3: 53–58.

    Article  CAS  Google Scholar 

  22. Kazunga, C., M. D. Aitken, and A. Gold (1999) Primary product of the horseradish peroxidase-catalyzed oxidation of pentachlorophenol.Environ. Sci. Technol. 33: 1408–1412.

    Article  CAS  Google Scholar 

  23. Hillgren, A., H. Evertsson, and M. Alden (2000) Interaction between lactate dehydrogenase and Tween 80 in aqueous solution.Pharm. Res. 19: 504–510.

    Article  Google Scholar 

  24. Gong, P. F., J. H. Xu, Y. F. Tang, and H. Y. Wu (2005) Improved catalytic performance ofBacillus megaterium epoxide hydrolase in a medium containing Tween-80.Biotechnol. Prog. 19: 652–654.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, The University of Seoul, 130-743, Seoul, Korea

    E. Y. Kim

  2. Department of Urban Environment, Seoul Development Institute, 137-603, Seoul, Korea

    Y. J. Choi

  3. Department of Food and Biotechnology and Department of Innovative Industrial Technology, Hoseo University, 336-795, Asan, Korea

    H. J. Chae

  4. Department of Chemical and Process Engineering, University of Canterbury, Private Bag 4800, Christchurch, New Zealand

    K. H. Chu

Authors
  1. E. Y. Kim
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  2. Y. J. Choi
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  3. H. J. Chae
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  4. K. H. Chu
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Correspondence to E. Y. Kim.

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Kim, E.Y., Choi, Y.J., Chae, H.J. et al. Removal of aqueous pentachlorophenol by horseradish peroxidase in the presence of surfactants. Biotechnol. Bioprocess Eng. 11, 462–465 (2006). https://doi.org/10.1007/BF02932315

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  • DOI: https://doi.org/10.1007/BF02932315

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