Skip to main content
SpringerLink
Log in

Development of a test strip reader for a lateral flow membrane-based immunochromatographic assay

  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

A low-cost, simple strip reader system using a linear movement mechanism of CD-ROM deck has been developed to characterize a lateral flow membrane-based immunochromatographic assay. The test strip reader was assembled by a CD-ROM deck and home-made optical head especially designed for immunoassays. The optical head for detecting reflected light from the test strip surface consists of green light-emitting diode, large area silicon photodiode, and anodized aluminum mounting block providing a slit structure for cutting light from the LED. The stepping motor of the deck was operated in the full step mode, whose distance of each reading point is about 0.15 mm. The performance of the strip reader was tested by analysis of HBV (hepatitis B virus) antigen test kit. This strip reader can be useful for inexpensive, disposable, and membrane-based assays that provide visual evidence of the presence of an analyte in a liquid sample.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kasahara, Y. and Y. Ashihara (1997) Simple devices and their possible application in clinical laboratory downsizing.Clin. Chim. Acta 267: 87–102.

    Article  CAS  Google Scholar 

  2. Lee, K. S., T. H. Kim, M. C. Shin, W. Y. Lee, and J. K. Park (1999) Disposable liposome immunosensor for theophylline combining an immunochromatographic membrane and a thick-film electrode.Anal. Chim. Acta 380: 17–26.

    Article  CAS  Google Scholar 

  3. Cunningham, D. D. (2001) Fluidics and sample handling in clinical chemical analysis.Anal. Chim. Acta 429: 1–18.

    Article  CAS  Google Scholar 

  4. Li, Y., K. Chen, S. Wei, J. He, and J. Mu (1993) A novel immunoelectrode assay for hepatitis B surface antigen.Sens. Actuators B 12: 49–52.

    Article  CAS  Google Scholar 

  5. Yokota, M., T. Mitani, H. Tsujita, T. Kobayashi, T. Higuchi, A. Akane, and M. Nasu (2001) Evaluation of prostate-specific antigen (PSA) membrane test for forensic examination of semen.Legal Med. 3: 171–176.

    Article  CAS  Google Scholar 

  6. Reddy, J. R., J. Kwang, K. F. Lechtenberg, N. C. Khan, B. P. Reddy, and M. M. Chengappa (2002) An immunochromatographic serological assay for the diagnosis ofMycobacterium tuberculosis.Comp. Immun. Microbiol. Infect. Dis. 25: 21–27.

    Article  CAS  Google Scholar 

  7. Shyu, R. H., H. F. Shyu, H. W. Liu, and S. S. Tang (2002) Colloidal gold-based immunochromatographic assay for detection of ricin.Toxicon 40: 255–258.

    Article  CAS  Google Scholar 

  8. Watanabe, H., A. Satake, Y. Kido, and A. Tsuji (2001) Monoclonal-based enzyme-linked immunosorbent assay and immunochromatographic rapid assay for salinomycin.Anal. Chim. Acta 437: 31–38.

    Article  CAS  Google Scholar 

  9. Valecha, N., V. P. Sharma, and C. U. Devi (1998) A rapid immunochromatographic test (ICT) for diagnosis ofPlasmodium falciparum.Diagn. Microbiol. Infect. Dis. 30: 257–260.

    Article  CAS  Google Scholar 

  10. Jelinek, T., L. Amsler, M. P. Grobusch, and H. D. Nothdurft (1999) Self-use of rapid tests for malaria diagnosis by tourists.The Lancet 354: 1609.

    Article  CAS  Google Scholar 

  11. Miwa, H., S. Akamatsu, T. Tachikawa, T. Sogabe, K. Ohtaka, A. Nagahara, Y. Sugiyama, and N. Sata (2001) On-site diagnosis of H. pylori infection by urine.Diagn. Microbiol. Infect. Dis. 39: 95–97.

    Article  CAS  Google Scholar 

  12. An, C. D., T. Yoshiki, G. Lee, and Y. Okada (2001) Evaluation of a rapid qualitative prostate specific antigen assay, the one step PSATM test.Cancer Lett. 162: 135–139.

    Article  Google Scholar 

  13. Chen, R., T. M. Li, H. Merrick, R. F. Parrish, V. Bruno, A. Kwong, C. Stiso, and D. J. Litman (1987) An internal clock reaction used in a one-step enzyme immunochromatographic assay of theophylline in whole blood.Clin. Chem. 33: 1521–1525.

    CAS  Google Scholar 

  14. Locascio-Brown, L., A. L. Plant, V. Horváth, and R. A. Durst (1990) Liposome flow injection immunoassay: Implications for sensitivity, dynamic range, and antibody regeneration.Anal. Chem. 62: 2587–2593.

    Article  CAS  Google Scholar 

  15. Herr, J. C., K. L. Klotz, P. Anderson, E. Adams, N. Moore, and S. Howards (1999) Progress in developing an immunochromatographic device for sperm detection.Clin. Immunol. Newslett. 19: 52–58.

    Article  Google Scholar 

  16. Waters, L. C., R. W. Counts, A. Palausky, and R. A. Jenkins (1995) Colorimetric strip tests: a comparison of visual and reflectometric measurements for quantitative applications.J. Hazardous Materials 43: 1–12.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bio Electronics Group, LG Electronics Institute of Technology, 137-724, Seoul, Korea

    Suhyeon Kim

  2. Department of BioSystems, Korea Advanced Institute of Science and Technology, 305-701, Daejeon, Korea

    Je-Kyun Park

Authors
  1. Suhyeon Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Je-Kyun Park
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Je-Kyun Park.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, S., Park, JK. Development of a test strip reader for a lateral flow membrane-based immunochromatographic assay. Biotechnol Bioproc E 9, 127–131 (2004). https://doi.org/10.1007/BF02932995

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02932995

Keywords

Search

Navigation