Skip to main content
SpringerLink
Log in

Application clinique de la cytométrie de flux de I’ADN à la caractérisation des polypes colorectaux

Clinical application of DNA flow cytometry for characterization of colorectal polyps

  • Published:
Acta Endoscopica

Résumé

Nonante-huit polypes adénomateux ont été biopsiés en cours de coloscopie. Les biopsies ont été étudiées par la méthode de cytométric de flux de l’ADN. Les résultats ont été rapportés à la taille, au type ďimplantation et à l’histopathologie des polypes. Tous les adénomes de type strictement tubulaire sont diploîdes. Les anomalies chromosomiales grossières se traduisant par une distribution de l’ADN de type aneuploîde, sont observées dans 31% des adénomes adénovilleux ou villeux. Elles sont présentes dans les polypes de toute taille et měme dans les cas où une dégénérescence maligne n’a pas été suspectée par l’examen histopathologique. La cytométric de flux de l’ADN peut, pour cette raison, ětre utilisée dans le diagnostic précoce de la transformation maligne. Les résultats suggèrent que même les polypes de petite taille devraient être systématiquement enlevés par coloscopie.

Summary

Ninety-eight adenomatous polyps have been biopsied at colonoscopy. The biopsies have been studied by means of DNA flow cytometry. The results have been related to polyp size, type of polyp attachment and to histopathology. All strictly tubular adenomas were diploid. Gross chromosomal aberrations reflected in aneuploid DNA patterns were found in 31% of the tubulo-villous or villous adenomas. They were present in polyps of all sizes and even in cases without suspicion of malignancy according to histopathology. DNA flow cytometry may therefore be used for early diagnosis of malignant transformation. The results suggest that also small polyps should be removed when found at colonoscopy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Références

  1. GILLESPIE P.E., CHAMBERS T.J., CHAN K.W., DORONZO F., MORSON B.C., WILLIAMS C.B. — Colonic adenomas — a colonoscopy survey.Gut, 1979,20, 240–245.

    Article  PubMed  CAS  Google Scholar 

  2. Goligher J. —Surgery of the Anus, Rectum and Colon. Baillière Tindann, fifth edition, 1984.

  3. GRINNELL R.S., LANE N. — Benign and malignant adenomatous polyps and papillary adenomas of the colon and rectum. An analysis of 1,856 tumors in 1,335 patients.Int. Abstracts of Surgery, 1958,106, 519–538.

    CAS  Google Scholar 

  4. HAMMARBERG C., SLEZAK P., TRIBUKAIT B. — Early detection of malignancy in ulcerative colitis. A flowcytometric DNA study.Cancer, 1984,53, 291–295.

    Article  PubMed  CAS  Google Scholar 

  5. Morson B.C., Sobin L.H.Histological typing of intestinal tumours. International Histological Classification of Tumours. No. 15. WHO, Geneva, 1976.

  6. MORSON B.C. —Polyps and cancer of the large bowel. In: Yardley, Morson, Abbell, The gastrointestinal tract. International academy of Pathology Monograph 18 (Williams & Wilkins, Baltimore, 1977).

    Google Scholar 

  7. MORSON B.C. —The pathogenesis of colorectal cancer. In: Bennington, Major problems in pathology, vol. 10 (Saunders, Philadelphia, 1978).

    Google Scholar 

  8. TRIBUKAIT B., HAMMARBERG C., RUBIO C. — Ploidy and proliferation pattern in colo-rectal adenocarcinomas related to Dukes’ classification and to histopathological differentiation.Acta path. microbiol. immunol. scand., Sect. A, 1983,91, 89–95.

    CAS  Google Scholar 

  9. Tribukait B. —Flow cytometry in surgical pathology and cytology of tumors of the genito-urinary tract. In: Koss L. G., Coleman D. V. (eds) Advances in Clinical Cytology, vol. 2. Masson Publ. U.S.A, Inc., 1984, 163–189.

  10. TRIBUKAIT B., HAMMARBERG C. — DNA flow cytometry of endoscopic biopsies for detection of malignancy in ulcerative colitis. A study based on the DNA patterns of gastro-intestinal tumours.Acta Endoscopica in press, 1985,15, 257–264.

    Article  Google Scholar 

  11. WEISS H., WILDNER G. P., JACOBASCH K.H., HEINZ U., SCHAELICKE W. — Characterization of human adenomatous polyps of the colorectal bowel by means of DNA distribution pattern.Oncology, 1985,42, 33–41.

    Article  PubMed  CAS  Google Scholar 

  12. SANDBERG A. A. —The Chromosomes in Human Cancer and Leukemia (Elsevier, New York, 1980).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Surgery, Karolinska Hospital, S-104 01, Stockholm, Sweden

    C. Hammarberg

  2. Dept. of Medical Radiobiology, Karolinska Institute, Stockholm, Sweden

    B. Tribukait

  3. Dept of Pathology, Karolinska Hospital, Stockholm, Sweden

    C. Rubio

  4. Dept of Radiodiagnostics, Karolinska Hospital, Stockholm, Sweden

    P. Slezak

Authors
  1. C. Hammarberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Tribukait
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Rubio
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Slezak
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Tirés à part: Professor. B. Tribukait Dept. of Medical Radiobiology, Box 60204, S-104 01 Stockholm(Sweden).

Cette étude a été accomplie avec le soutien de la Stockholm Cancer Society, du Karolinska Institute et des Fondations Bergwall, Juhlin et Wallström.

This research was supported by grants from the Stockholm Cancer Society, the Karolinska Institute, the Bergwall, Juhlin, and Wallström Foundations.

About this article

Cite this article

Hammarberg, C., Tribukait, B., Rubio, C. et al. Application clinique de la cytométrie de flux de I’ADN à la caractérisation des polypes colorectaux. Acta Endosc 15, 265–273 (1985). https://doi.org/10.1007/BF02966119

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02966119

Mots-clés

Key-words

Search

Navigation