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DNA Molecular profiling: a new approach to quality control of chinese drugs

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Conclusions

DNA technology based molecular profiling provides the ultimate and definitive means of authentication for Chinese drugs. RFLP, RP-PCR and microsatellite-SSR examine the whole genome. With the appropriate primer, it is in principle possible to differentiate closely related species and even one individual from another. PCR-SR, PCR-RFLP, AFLP, SCAR, DALP and DNA sequencing examine a defined region on the genome and produce well-defined conclusions. Since different regions on the genome evolve to different extents, by choosing the target DNA carefully, organisms of different systematic levels can be compared. Of these molecular techniques mentioned above, genomic fingerprints and DNA sequences are equally successful in identification of Chinese drugs. Although RFLP, RP-PCR methods do not require prior to genetic background of the genome and can detect DNA polymorphism, the reliability of these fingerprint results can be greatly influenced by experimental conditions. DNA sequencing method focuses on a defined locus on the genome and may provide a definitive means for authentication of Chinese drugs. We, therefore, conclude that it is essential to combine genomic fingerprinting with DNA sequencing analysis in order to increase the accuracy of quality control.

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Authors and Affiliations

  1. Institute of Chinese Materia Medica, China Academy of TCM, 100700, Beijing

    Hui Cao & Yuping Liu

  2. Toyama Medical and Pharmaceutical University, Japan

    Komatsu Katsuko

  3. The Chinese University of Hong Kong, HKSAR

    But Paul Pui-Hay & Shaw Pang-Chui

  4. Now are in the Toyama Medical and Pharmaceutical Univeristy, Japan

    Hui Cao & Yuping Liu

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  1. Hui Cao
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  5. Shaw Pang-Chui
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Cao, H., Liu, Y., Komatsu, K. et al. DNA Molecular profiling: a new approach to quality control of chinese drugs. CJIM 6, 71–75 (2000). https://doi.org/10.1007/BF02973161

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