Skip to main content
SpringerLink
Log in

Human renal dipeptidase from kidneys of renal stone patients: Partial purification

  • Research Article
  • Published:
Archives of Pharmacal Research Aims and scope Submit manuscript

Abstract

Human renal dipeptidase (RDPase) was purified from surgically removed kidneys of renal stone patients by affinity chromatography using its specific inhibitor, cilastatin, as the ligand. The partial purified RDPase of 6 mg exhibited specific activity of 99.4 unit/mg with 2,029 fold purification. It was composed of a slow moving major band (96%) and a fast moving minor band (4%). The minor band was not a contaminant as it showed a dipeptidase-specific activity. The kinetic parameters determined with glycyldehydrophenylalanine (Gdp) as synthetic substrate were Vmax, 322.6 μmol/min/mg and Km, 0.102 mM. This experiment provided biochemical evidences that surgically removed, nonfunctional kidneys in respect of glomerular filtration still retained high activity of renal dipeptidase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References Cited

  • Adachi, H., Katayama, T., Inuzuka, C. and Oikawa, S., Tsujimoto, M. and Nakazato, H. Identification of membrane anchoring site of human renal dipeptidase and construction and expression of a cDNA for its secretory form.J. Biol. Chem., 265, 15341–15345 (1990).

    PubMed  CAS  Google Scholar 

  • Armstrong, D. J., Mukhopadhyay, S. K. and Campbell, B. J., Physicochemical characterization of renal dipeptidase.Biochem., 13(8), 1745–1750 (1974).

    Article  CAS  Google Scholar 

  • Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding.Anal. Biochem., 72, 248–254 (1976).

    Article  PubMed  CAS  Google Scholar 

  • Campbell B. J., Renal dipeptidase.Methods Enzymol., 19, 722–729 (1970).

    Article  Google Scholar 

  • Campbell, B. J., Forrester, L. J., Zahler, W. L. and Burks, M., β-lactamase activity of purified and partially characterized human renal dipeptidase.J. Biol. Chem., 259, 14586–14590 (1984).

    PubMed  CAS  Google Scholar 

  • Campbell, B. J., Lin, Y. C. and Bird, M. E., Renal aminopeptidase and copper-activated peptide hydrolysis.J. Biol. Chem., 238, 3632–3640 (1963).

    PubMed  CAS  Google Scholar 

  • Campbell, B. J., Shih, Y. D., Forrester, L. J. and Zahler, W. L., Speci-ficity and inhibition studies of human renal dipeptidase.Biochem. Biophys. Acta, 956, 110–118 (1988).

    PubMed  CAS  Google Scholar 

  • Campbell, B. J., Yudkin, W. H. and Klotz, I. M., Spectorphotometric properties of an intermediate in the enzymic hydrolysis of glycyldehydrophenylalanine.Archiv. Biochem. Biophys., 92, 257–263 (1961).

    Article  CAS  Google Scholar 

  • Farrell, C. A., Allegetto, N. J. and Hitchcock, M. J. M., Cilastatinsensitive dehydrogenase I enzymes from three sources all catalyze carbapenem hydrolysis and conversion of leukotriene D4 to leukotriene E4.Archiv. Biochem. Biophys., 256, 253–259 (1987).

    Article  CAS  Google Scholar 

  • Hirota, T., Nishikawa, Y., Tanaka, M., Fukuda, K., Igarashi, T. and Kitagawa, H., Localization of dehydropeptidase-I, an enzyme processing glutathione, in the rat kidney.J. Biochem., 102, 547–550 (1987).

    PubMed  CAS  Google Scholar 

  • Hooper, N. M. and Turner, A. J., Ectoenzymes of the kidney microvillar membrane.Biochem. J., 261, 811–818 (1989).

    PubMed  CAS  Google Scholar 

  • Horadam, V. W., Smilack, J. D., Montgomery, C. L. and Werringloer, J.,In vitro activity of N-formimidoyl thienamycin (MK0787), a crystalline derivative of thienamycin.Antimicrob. Agent. Chemother. 18, 557–561 (1980).

    CAS  Google Scholar 

  • Kahan, F. M., Kropp, H., Sundelof, J. G. and Birnbaum, J., Thienamycin: development of imipenem-cilastatin.J. Antimicrob. Chemother. 12, suppl. D: 1–35 (1983).

    PubMed  CAS  Google Scholar 

  • Kahan, J. S. Kahan, F. M., Goegelman, R, Currie, S. A., Jackson, M., Stapley, E. O., Miller, T. W., Miller, A. K., Hendlin, D., Mochales, S., Hernandez, S. and Woodruff, H. B., Thienamycin, A new β-lactam antibiotic. I. Discovery and isolation,16th Interscience Conference on Antimicrob. Agent Chemother, Chicago, Ill., Abstr. 227 (1976).

  • Kim, H. S. and Campbell, B. J., β-lactamase activity of renal dipeptidase aganist N-formimidoyl thienamycin.Biochem. Biophys. Res. Comm., 108, 1638–1642 (1982).

    Article  PubMed  CAS  Google Scholar 

  • Kim, H. S. and Campbell, B. J., Association of renal dipeptidase with the Triton-insoluble fraction of kidney microvilli.J. Memb. Biol., 75, 115–122 (1983).

    Article  CAS  Google Scholar 

  • Kropp, H., Sundelof, J. G., Hajdu, R. and Kahan, F. M., Metabolism of thienamycin and related carbapenem antibiotics by the renal dipeptidase: dehydropeptidase-l.Antimicrob. Agent. Chemother. 22, 62–70 (1982).

    CAS  Google Scholar 

  • Lewis, R. A., Austen, K. F. and Soberman, R. J., Leukotrienes and other products of the 5-lipoxygenase pathway; Biochemistry and relation to pathobiology in human diseasesN. Eng. J. Med., 323, 645–655 (1990).

    CAS  Google Scholar 

  • Littlewood, G. M., Hooper, N. M. and Turner, A. J., Ectoenzymes of the kidney microvillar membrane.Biochem. J., 257, 361–367 (1989).

    PubMed  CAS  Google Scholar 

  • Matsuda, K., Sasaki, K., Inoue, K., Kondo, H., Inoue, M. and Mitsuhashi, S.,In vitro antibacterial activity of Sch 34343 and its stability to β-lactamases and renal dehydropeptidase I, Antimicrob.Agent. Chemother., 28, 684–688 (1985).

    CAS  Google Scholar 

  • Mitsuhashi, S., Fuse, A., Mikami, H., Saino, Y. and Inoue, M., Purification and characterization of human renal dehydropeptidase I.Antimicrob. Agent. Chemother., 32, 587–588 (1988).

    CAS  Google Scholar 

  • Ornstein, L., Disc electrophoresis-I Background and theory.Annal. New York Acad. Sci., 121, 321–349 (1964).

    Article  CAS  Google Scholar 

  • Rene, A. M. and Campbell, B. J., Amino acid composition and effect of pH on the kinetic parameters of renal dipeptidase.J. Biol. Chem., 244, 1445–1450 (1969).

    PubMed  CAS  Google Scholar 

  • Sugiura, M., Yoshimasa, I. and Kazuyuki, H., Detection of dipeptidase and tripeptidase activities on polyacrylamide gel and cellulose acetate gel by the reduction of tetrazolium salts.Anal. Biochem., 81, 481–484 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Tally, F. P., Jacobus, N. V. and Gorbach, S. L.,In vitro activity of N-formimidoyl thienamycin (MK0787).Antimicorb. Agent. Chemother., 18, 642–644 (1980).

    CAS  Google Scholar 

  • Toda, M., Sato, K., Nakazawa, H., Inoue, M. and Mitsuhashi, S., Effect of N-formimidoyl thienamycin (MK 0787) on β-lactamases and activity against β-lactamase-producing strains.Antimicrob. Agent. Chemother. 18, 837–838 (1980).

    CAS  Google Scholar 

  • Welch, C. L. and Campbell, B. J., Uptake of glycine from L-alanylglycine into renal brush border vesicles.J. Memb. Biol., 54, 39–50 (1980).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Pharmacy, Chonnam National University, 300 Yongbong-dong, 500-757, Kwangju, Korea

    Haeng Soon Park, Doh -Ha Kim, Byung Ho Chung & Gyrung Soo Yoo

  2. Pathology Department, Westchester County Medical Center, NY10595, Valhalla, N.Y., USA

    Hyun S. Ellen Kwark

  3. Department of Internal Medicine, Medical School, Chonbuk National University, 560-182, Chonbuk, Korea

    Sung Kwang Park & Sung Kyew Kang

Authors
  1. Haeng Soon Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Doh -Ha Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hyun S. Ellen Kwark
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sung Kwang Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sung Kyew Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Byung Ho Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Gyrung Soo Yoo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Park, H.S., Kim, D.H., Kwark, H.S.E. et al. Human renal dipeptidase from kidneys of renal stone patients: Partial purification. Arch. Pharm. Res. 16, 295–299 (1993). https://doi.org/10.1007/BF02977519

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02977519

Key words

Search

Navigation