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Preparation and performance of cellulose bead-entrapped whole cell glucose isomerase

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Abstract

A novel simple and mild technique to entrap glucose isomerase activeA. missouriensis cells within α-cellulose beads has been developed. Regenerated α-cellulose beads were shown by SEM to be highly loaded with microbial cells. Kinetics, high operational stability, reactor performance, and low cost of carrier showed the entrapped cell preparation to be suitable for continuous isomerization of 45% glucose solution. This paper was presented at the 4th Enzyme Engineering Conference, Bad Neuenahr, Germany. Sponsored by the Engineering Foundation, New York, New York.

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Authors and Affiliations

  1. Academy of Finland, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo 15, Finland

    Yu-Yen Linko

  2. Department of Chemistry, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo 15, Finland

    Risto Viskari, Leila Pohjola & Pekka Linko

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  1. Yu-Yen Linko
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  2. Risto Viskari
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  3. Leila Pohjola
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  4. Pekka Linko
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Linko, YY., Viskari, R., Pohjola, L. et al. Preparation and performance of cellulose bead-entrapped whole cell glucose isomerase. Journal of Solid-Phase Biochemistry 2, 203–212 (1977). https://doi.org/10.1007/BF02996742

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  • DOI: https://doi.org/10.1007/BF02996742

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