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Growth of Tsuru-rindo (Tripterospermum japonicum) culturedin vitro under various sources of light-emitting diode (LED) irradiation

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Abstract

We studied the effects of light generated by LEDs on the growth of Tsururindo (Tripterospermum japonicum) shoots.

Apical shoots (2–3 cm long) were cultured on MS basal media supplemented with 3% sucrose, and were maintained for four weeks under five different light qualities: F (fluorescent lamp), red LED (R), 70% red + 30% blue LED (R7B3), 50% red + 50% blue (R5B5), or blue LED (B). Rooting was promoted by red light (100%) but was inhibited by blue light. Plant growth, as defined by root number, fresh weight, and chlorophyll content, was generally healthier for cultures irradiated with mixed LEDs, and was the best under R7B3. Ventilation resulted in more rapid apical shoot growth and rooting compared with control plants, when both were treated with the R7B3 system. We demonstrated here that plant growth can be controlled by using LEDs to adjust for the most effective irradiation conditions, compared with the performance observed when conventional fluorescent lamps are utilized.

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Literature cited

  • Bang JW, Lee MK, Chung SH (1994) Somatic embryogenesis and plant regeneration in leaf expiant cultures ofGentiana scabra var. buergeri. Kor J Plant Tiss Cult21: 233–237

    Google Scholar 

  • Blanke MB, Belcher AR (1989) Stomata of apple leaves culturedin vitro. Plant Cell Tiss Org Cult19: 85–89

    Article  Google Scholar 

  • Bula RJ, Morrow TW, Tibbitts TW, Barta DJ, Ignatius RW, Martin TS (1991) Light-emitting diodes as a radiation source for plants. Hort Sci26: 203–205

    CAS  Google Scholar 

  • Capellades MR, Fontarnau C, Debergh P (1990) Environment influences anatomy of stomata and epidermal cells in tissue-culturedRosa multiflora. J Amer Soc Hort Sci115: 141–145

    Google Scholar 

  • Casano S (1995) Effetti della qualita della luce sulla morfogenesi di specie arboree da frutto coltivatein vitro. Ph.D. thesis. University of Pisa, Pisa

    Google Scholar 

  • Chee R (1986)In vitro culture ofVitis: The effect of light spectrum, manganese sulfate and potassium iodide on morphogenesis. Plant Cell Tiss Org Cult7: 121–134

    Article  CAS  Google Scholar 

  • Chee R, Pool RM (1989) Morphogenic responses to propagule trimming, spectral irradiance, and photoperiod of grapevine shoots reculturedin vitro. J Amer Soc Hort Sci114: 350–354

    Google Scholar 

  • Cho MS, Chang JJ, Kwon ST (1992) Effects of culture conditions on micropropagation ofGentiana axillariflora var. coreana. Kor J Plant Tiss Cult19: 357–362

    Google Scholar 

  • Dooley JH (1991) Influence of lighting spectra on plant tissue culture. ASAE (American Society of Agricultural Engineers) Meeting, Chicago, Paper No. 917530

  • Fal MA, Majada JP, Gonzalez A, Tames RS (1999) Differences betweenDianthus caryophyllus L. cultivars inin vitro growth and morphogenesis are related to their ethylene production. Plant Growth Reg27: 131–136

    Article  CAS  Google Scholar 

  • Fuernkranz HA, Novak CA, Maynard CA (1990) Light effects onin vitro adventitious root formation in axillary roots of maturePrunus serotina. Physiol Plant80: 37–341

    Article  Google Scholar 

  • Gonzalez A, Arigita L, Majada J, Tames RS (1997) Ethylene involvement inin vitro organogenesis and plant growth ofPopulus tremula L. Plant Growth Reg22: 1–6

    Article  CAS  Google Scholar 

  • Kim NY, Kang TH, Kim DH, Kim YC (1999) A nitric oxide synthesis inhibitor from the roots ofGentiana scabra in RAW 264.7 cella. Kor J Phamacogn30: 173–176

    CAS  Google Scholar 

  • Kim SJ, Hahn EJ, Heo JW, Paek KY (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantletsin vitro.Sci Horticult101: 143–151

    Article  Google Scholar 

  • Liao Z, Chen M, Tan F, Sun X, Tang K (2004) Micropropagation of endangered Chinese aloe. Plant Cell Tiss Org Cult76: 83–86

    Article  CAS  Google Scholar 

  • Lim JD, Kim MJ, Yu CY (2000) Induction and RAPD analysis of mutant plants by chemical mutagens inGentiana axillariflora Leveille. Kor J Plant Tiss Cult27: 89–94

    Google Scholar 

  • Malabadi RB, Mulgund GS, Nataraja K (2004) Efficient regeneration ofVanda coerulea, an endangered orchid, using thidiazuron. Plant Cell Tiss Org Cult76: 289–293

    Article  CAS  Google Scholar 

  • Mccartan SA, Staden JV (2003) Micropropagation of the endangeredKniphofia leucocephala Baijnath. In Vitro Cell Dev Biol Plant39: 496–499

    Article  Google Scholar 

  • McCree KJ (1972) The action spectra, absorptance and quantum yield of photosynthesis in crop plants. Agr Met9: 191–196

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant15: 473–497

    Article  CAS  Google Scholar 

  • Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Responses of strawberry plantlets culturedin vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell Tiss Org Cult73: 43–52

    Article  CAS  Google Scholar 

  • Paek KY, Hahn EJ (2000) Cytokinins, auxins and activated charcoal affect organogenesis and anatomical characteristics of shoot tip cultures ofLisianthus [Eustoma grandiflorum (Raf.)]. In Vitro Cell Dev Biol Plant36: 128–132

    Article  CAS  Google Scholar 

  • Pinker I, Zoglauer K, Goring H (1989) Influence of light on adventitious root formation in birch shoot culturesin vitro. Biol Plant31: 254–260

    Article  Google Scholar 

  • Prasad TK, Cline MG (1985a) Shoot inversion-induced ethylene inPharbitis nil induces the release of apical dominance by restricting shoot elongation. Plant Sci38: 163–172

    Article  PubMed  CAS  Google Scholar 

  • Prasad TK, Cline MG (1985b) Mechanical perturbationinduced ethylene releases apical dominance inPharbitis nil by restricting shoot growth. Plant Sci41: 217–222

    Article  PubMed  CAS  Google Scholar 

  • Rossi F, Baraldi R, Facini O, Lercari B (1993) Photomorphogenic effects onin vitro rooting of Prunus rootstock GF 655-2. Plant Cell Tiss Org Cult32: 145–151

    Article  CAS  Google Scholar 

  • Sabo A, Krekling T, Appelgren M (1995) Light quality affects photosynthesis and leaf anatomy of birch plantletsin vitro. Plant Cell Tiss Org Cult41: 177–185

    Article  Google Scholar 

  • SAS (1989) SAS/STAT User’s Guide, Version 6, Ed 4, Vol 2. SAS Institute, Cary

    Google Scholar 

  • Schuerger AC, Brown CS, Stryjewski EC (1997) Anatomical features of pepper plants (Capsicum annuum L.) grown under red light-emitting diodes supplemented with blue or far-red light. Ann Bot79: 273–282

    Article  PubMed  CAS  Google Scholar 

  • Seong NS, Park CH, Kim KS, Lee ST, Chang YH (1995)In vitro variant induction and its content of Gentiopicroside ofGentiana scabra Bunge. Kor J Med Crop Sci3: 40–44

    Google Scholar 

  • Seong NS, Park CH, Lee ST, Kim SM (1993) Plant regeneration and multiplication ofGentiana scabra Bunge through leaf and stem culture. Kor J Med Crop Sci1: 129–136

    Google Scholar 

  • Tibbitts TW, Morgan DC, Warrington IJ (1983) Growth of lettuce, spinach, mustard and wheat plants under four combinations of high-pressure sodium, metal halide and tungsten halogen lamps at equal PPFD. J Amer Soc Hort Sci108: 622–630

    Google Scholar 

  • Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol144: 307–313

    CAS  Google Scholar 

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Correspondence to Heutig Kyu Moon.

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Moon, H.K., Park, SY., Kim, Y.W. et al. Growth of Tsuru-rindo (Tripterospermum japonicum) culturedin vitro under various sources of light-emitting diode (LED) irradiation. J. Plant Biol. 49, 174–179 (2006). https://doi.org/10.1007/BF03031014

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  • DOI: https://doi.org/10.1007/BF03031014

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